# Core B - Transfection Core Laboratory

> **NIH NIH P01** · PENNSYLVANIA STATE UNIV HERSHEY MED CTR · 2020 · $165,390

## Abstract

PROJECT SUMMARY/ABSTRACT – CORE B: TRANSFECTION CORE LABORATORY
An important objective of the P01 research proposal is to examine the mechanisms by which the exaggerated
exercise pressor reflex (following femoral occlusion) is regulated by pro-inflammatory cytokines (IL-6 and TNF-
α), NaV1.7 and NaV1.8 channels, purinergic (P2X3) channels, prostaglandin (EP4) receptors, and acid sensing
ion channels (ASICs) expressed in dorsal root ganglion (DRG) neurons. The responses to contraction of
afferents following acute knockdown of myophosphorylase in gastrocnemius muscle will also be investigated.
Through Projects 2 and 3, the roles of these channels, receptors and signaling elements will be defined. In
order to achieve these aims, the Transfection Core will provide key help through three objectives: 1) the Core
will design effective siRNA sequences and provide cDNA clones that code for these `short hair' nucleotide
sequences; 2) the Core will perform the in vivo and in vitro transfection of DRG (L4-L5) and gastrocnemius
muscle; and 3) the Core will verify protein knockdown employing quantitative RT-PCR (mRNA levels) and
Western blotting (protein quantification). These three aims will assist Projects 2 and 3 in testing their specific
hypotheses. Overall, the work will lead to a better understanding of the role these proteins play in the
exaggerated exercise pressor reflex observed in peripheral artery disease (PAD).

## Key facts

- **NIH application ID:** 9857646
- **Project number:** 5P01HL134609-04
- **Recipient organization:** PENNSYLVANIA STATE UNIV HERSHEY MED CTR
- **Principal Investigator:** Victor Ruiz-Velasco
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $165,390
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9857646

## Citation

> US National Institutes of Health, RePORTER application 9857646, Core B - Transfection Core Laboratory (5P01HL134609-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9857646. Licensed CC0.

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