# Integration of Mechanical Forces and Signaling in the Morphogenesis of the Gut

> **NIH NIH R01** · HARVARD MEDICAL SCHOOL · 2020 · $411,458

## Abstract

ABSTRACT
The broad goal of this work is to understand morphogenesis of the vertebrate embryo and, in particular, to
understand the formation of the vertebrate gut. The hope is that the knowledge we glean will ultimately provide
insights into the developmental defects leading to congenital malformations. There has been great progress in
recent years in understanding the molecular cues orchestrating gut development. However, relatively less
attention has been given to the roles of physical forces in shaping the tissues of the gut. This study attempts to
identify the mechanical factors involved in the initial formation of the early gut, and to integrate the
understanding of those forces with the activity of signaling molecules that mediate the process. We will start by
defining the physical basis of cell movements during hind gut formation. Live imaging microscopy will be used
to define the kinematics of cell movement in the gut. Physical experiments will be used to determine the
location and directionality of mechanical forces in the endoderm. Fibroblast growth factors are key signals
directing cell movements of other tissues and the posterior embryo at the same time as the hindgut is forming.
Molecular and genetic perturbations will be used to establish the effects of Fgf signaling on the kinematics of
cell movement in the forming gut and to determine the pathways through which Fgf signaling acts in this tissue.
Finally, a computational model will be used to gain a quantitative understanding of the mechanics of gut tube
formation. Together, this work will substantially increase our understanding of the key initial steps by which the
vertebrate gut is formed.

## Key facts

- **NIH application ID:** 9861252
- **Project number:** 5R01HD089934-04
- **Recipient organization:** HARVARD MEDICAL SCHOOL
- **Principal Investigator:** CLIFFORD J. TABIN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $411,458
- **Award type:** 5
- **Project period:** 2017-02-01 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9861252

## Citation

> US National Institutes of Health, RePORTER application 9861252, Integration of Mechanical Forces and Signaling in the Morphogenesis of the Gut (5R01HD089934-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9861252. Licensed CC0.

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