# Molecular Pathology Shared Resource

> **NIH NIH P30** · UNIVERSITY OF COLORADO DENVER · 2020 · $315,958

## Abstract

MOLECULAR PATHOLOGY SHARED RESOURCE (Core-957)
ABSTRACT
Overview: The Molecular Pathology Shared Resource (MPSR) consists of 3 thematically related and
operationally coordinated business units (Histology, Cytogenetics and Pre-analytical Evaluation and Molecular
Testing (PEMT)). MPSR supports translational and clinical research projects of UCCC members by providing
clinical-grade services and molecular assays for analysis of pre-clinical and patient samples at reduced prices,
greatly facilitating cancer research. Equipment: Major equipment: Histology unit has tissue processors and
embedding stations, microtomes and automated staining equipment; Cytogenetics unit has high-quality Zeiss
fluorescence microscopes equipped with last generation cameras and automated interference filter wheels;
PEMT has Next Generation Sequencers (NGS), capillary electrophoresis, real-time PCR among others.
Services: MPSR offers pathologist examination of tissue samples, tumor sample microdissection, standard and
customizable histologic tissue processing and staining, cytogenetic techniques (ploidy analyses, G-banding and
spectral karyotyping, metaphase FISH with single locus and painting probes, interphase FISH using 2-target to
6-target multiplexing), and a wide range of nucleic acid-based assays (Sanger sequencing, next-generation
sequencing, quantitative PCR, micro-satellite instability testing). Activities in the Histology and PEMT units are
performed in a CLIA-certified, CAP-inspected environment by dedicated technologists, resulting in clinical-grade
data while those in Cytogenetics are GLP. All data is provided in an analyzed format and include bioinformatic
analysis of complex data sets, coupled with comprehensive interpretation of results and illustrative
documentation. The MPSR also supports and aids in the design, implementation, and validation of custom
assays both for specific investigator use and for wider use by the UCCC community. Consultation and
Education: The MPSR offers consultation, as well as one-on-one and group educational sessions for all services
and assays, for both assay design and data analysis. Instructional sessions are offered to UCCC members, in
order for users to precisely understand which assays may best serve their individual translational research
needs. Management: The MPSR is a UCCC shared resource managed by the Cancer Center. CCSG funding
represents 21% of the annual operating budget. The remaining support comes from user fees (79%). MPSR is
overseen by UCCC Associate Director for Translational Research. Use of Services: Since July 2011, 147
investigators have used the services. 78% percent of users were UCCC members, representing all 6 Programs
and resulting in 118 publications. Future Directions: The MPSR has a number of future directions that will
enhance the SR and UCCC member cancer research: 1) The PEMT unit will significantly expand NGS
capabilities, increasing the number of genes analyzed in panels and the types of altera...

## Key facts

- **NIH application ID:** 9867667
- **Project number:** 5P30CA046934-32
- **Recipient organization:** UNIVERSITY OF COLORADO DENVER
- **Principal Investigator:** Dan Theodorescu
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $315,958
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9867667

## Citation

> US National Institutes of Health, RePORTER application 9867667, Molecular Pathology Shared Resource (5P30CA046934-32). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9867667. Licensed CC0.

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