# Animal Core

> **NIH NIH U19** · DUKE UNIVERSITY · 2020 · $203,809

## Abstract

The overall goals of the Animal Models Core is to support all programs of the IPCAVD by performing
immunization studies in DH270 unmutated common ancestor (UCA) VH + VL knock-in (KI) mice with V3- Man9
glycopeptide primes and modified mRNA boosts (Project 1, Aim 2), by performing immunization studies with
the optimized V3-Man9 glycopeptide and modified mRNA regimen in rhesus macaques (Project 1, Aim 3),
and by performing immunization studies in DH270 UCA VH + VL KI mice and overseeing toxicity studies of
CGMP clinical trials material (CTM) mRNAs in collaboration with Project 2. The Specific Aims of this Animal
Models Core will include:
Aim 1. Perform immunization studies in DH270 unmutated common ancestor (UCA) VH + VL knock-in
(KI) mice with Man9-V3 glycopeptides and modified mRNAs in Project 1, Aim 2.
Aim 2. Perform immunization with the optimized Man9-V3 glycopeptide and modified mRNA regimen in
rhesus macaques (Project 1, Aim 3).
Aim 3. Perform immunization studies in DH270 UCA VH + VL KI mice and overseeing toxicity studies of
CGMP clinical trials material (CTM) mRNAs in collaboration with
This project will advance nucleoside-modified mRNA as a platform for HIV-1 Env immunization and establish
its immunogenicity in humanized mice and NHPs. Preliminary studies have demonstrated that the
extraordinary immunogenicity of modified mRNAs in mice translates to rhesus macaques in the setting of Zika
and HIV immunizations. Thus, we anticipate that mRNA will be both cost-effective and highly immunogenic and
therefore have the potential to be transformative to the HIV vaccine field as a vector.

## Key facts

- **NIH application ID:** 9868264
- **Project number:** 5U19AI135902-03
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Barton F. Haynes
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $203,809
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9868264

## Citation

> US National Institutes of Health, RePORTER application 9868264, Animal Core (5U19AI135902-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9868264. Licensed CC0.

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