# Analytic Microscopy Core

> **NIH NIH P30** · H. LEE MOFFITT CANCER CTR & RES INST · 2020 · $94,672

## Abstract

PROJECT SUMMARY
The Analytic Microscopy Core (AMC) Facility was established in 1999 to provide MCC members with access to
equipment and technical expertise that are needed to perform high-level microscopy experiments. These
services allow members to visualize and quantify complex cellular and sub-cellular processes in multiple
dimensions. The specific aims of the AMC are to provide: 1) assistance with study design for use of
microscopy, 2) access to advanced microscopy systems and image analysis platforms, and 3) training on use
of AMC's microscopy systems and image analysis technologies. State-of-the-art microscopy equipment
located in the AMC includes confocal, wide-field fluorescence, multiphoton, stereo, live cell, intravital, whole
slide scanning, and laser capture microdissection (LCM) microscopy systems. The AMC's image analysis
capabilities include image quantification, automated segmentation, co-localization, 3D rendering, motion
tracking, and deconvolution. The AMC is staffed by four full-time specialists who are skilled in both microscopy
and cancer-related research fields, and it is led by Marilyn Bui, MD, PhD (Scientific Director), who is an
American Board of Pathology-certified pathologist with extensive experience in cancer research and digital
pathology. The AMC staff work closely with members to design, image, and analyze experiments, allowing
members to obtain high-quality and reproducible data. Further, the AMC collaborates with other Shared
Resource Facilities at MCC to manage complex projects and to ensure proper handoffs of materials and data.
Given the costs of modern microscopy systems and the logistics involved with outsourcing microscopy
experiments, the services provided by the AMC are an essential resource for MCC members. Over the past
five years, the AMC has experienced a 50% increase in usage, supporting 154 publications. During the most
recent fiscal year, the AMC supported 58 members, and 83% of total usage was for peer-review-funded
members. Since the last review, the AMC has added several new microscopy systems, including wide-field
fluorescence, confocal, multiphoton, LCM, and three live cell imaging platforms. These new technologies have
sustained high levels of usage at the AMC and have provided members with the most advanced microscopy
platforms and services. Moving forward, the AMC will continue to evaluate and provide state-of-the-art
equipment and services to meet the future microscopy needs of MCC members.

## Key facts

- **NIH application ID:** 9868906
- **Project number:** 5P30CA076292-22
- **Recipient organization:** H. LEE MOFFITT CANCER CTR & RES INST
- **Principal Investigator:** Marilyn M Bui
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $94,672
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9868906

## Citation

> US National Institutes of Health, RePORTER application 9868906, Analytic Microscopy Core (5P30CA076292-22). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9868906. Licensed CC0.

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