# Cell Function Analysis Core

> **NIH NIH P30** · UNIVERSITY OF WASHINGTON · 2020 · $282,422

## Abstract

The overall objective of the Cell Function Analysis Core at the University of Washington Diabetes Research
Center is to provide affiliates with analyses of glucose metabolism, mitochondrial function and intracellular
signaling to support research of diabetes, obesity and related disorders. To achieve this goal, the Core aims to:
(1) Provide real time functional analysis using in vitro flow culture systems of tissues/cells; (2) Provide in vivo
assessments of metabolic phenotypes in rodent models important in diabetes research; (3) Provide static
assessment of cellular metabolism and function; (4) Harvest, isolate and culture primary tissue from rodents,
including islets and islet cells, liver, retina and brain, for subsequent morphological and functional
characterization, as well as to procure human islets for the same purposes; (5) Offer training and consultation
to affiliates, their trainees and staff; and (6) Develop new analytical tools requested by affiliates to support their
studies of the metabolic regulation of cell function as it relates to research in diabetes, obesity and related
disorders.
 Since inception of the Core in 2002, in vitro analysis has been the major focus. Cell and tissue types that
have been analyzed include islets, retina, skeletal muscle, stem cells, macrophages, lymphocytes, adipocytes,
endothelial cells, neuronal cells and liver/hepatocytes. Recently, in vivo services have been added to combine
both the detailed and mechanistic analyses provided in cell and tissue studies with the ability to test the roles
of identified processes in whole body settings. Whole animal studies currently offered include glucose and
insulin tolerance tests, hyperinsulinemic-euglycemic clamps, islet transplantation and collection of lymph.
Expansion of new in vitro, ex vivo, and in vivo services has allowed the Core to better serve the needs of the
Center's research base. As diabetes affects metabolism and signaling in many cell types, the services of the
Cell Function Analysis Core continue to be of great value to many Center affiliate investigators. The Core plans
to continue to provide users with systematic and integrated approaches to the analysis of cell types that are
critically involved in diabetes and its complications, obesity and related disorders.

## Key facts

- **NIH application ID:** 9869000
- **Project number:** 5P30DK017047-44
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** IAN R SWEET
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $282,422
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9869000

## Citation

> US National Institutes of Health, RePORTER application 9869000, Cell Function Analysis Core (5P30DK017047-44). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9869000. Licensed CC0.

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