# GENOME WIDE MAPPING OF LOOPS USING IN SITU HI-C

> **NIH NIH UM1** · BAYLOR COLLEGE OF MEDICINE · 2020 · $947,809

## Abstract

GENOME WIDE MAPPING OF LOOPS USING IN SITU HI-C
Principal Investigator: Erez Lieberman Aiden
Project Summary
The roughly two meters of DNA in the human genome is intricately packaged to form
the chromatin and chromosomes in each cell nucleus. In addition to its structural role,
this organization has critical regulatory functions. In particular, the formation of loops in
the human genome plays an essential role in regulating genes. We recently
demonstrated the ability to create reliable maps of these loops, using an in situ Hi-C
method for three-dimensional genome sequencing. Hi-C characterizes the three-
dimensional configuration of the genome by determining the frequency of physical
contact between all pairs of loci, genome-wide. The proposed center will apply Hi-C and
other new technologies to characterize genomic loops, their regulation, and their
functions. We will specifically examine these structures in a wide variety of ENCODE
cell types. The principles deduced from our study will be applicable to any mammalian
tissue type.
Aim 1 will apply Hi-C to 80 cell types to generate maps of loops genome-wide. Aim 2
will combine Hi-C data with existing ENCODE data in order to identify the specific DNA
elements responsible for establishing each loop. Aim 3 will share these datasets
through our Juicebox data visualization system.
The proposed project will advance our understanding of the determinants and functions
of chromatin loops, provide a critical reference of loop structures in the human and
mouse genomes, and present a technological framework for comprehensive analysis of
genome folding in any cell type. It will also form a basis for studying the contribution of
3D genome structure to human disease and development.
All data will be freely and rapidly released to the scientific community.

## Key facts

- **NIH application ID:** 9872199
- **Project number:** 5UM1HG009375-04
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **Principal Investigator:** Erez Lieberman-Aiden
- **Activity code:** UM1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $947,809
- **Award type:** 5
- **Project period:** 2017-02-01 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9872199

## Citation

> US National Institutes of Health, RePORTER application 9872199, GENOME WIDE MAPPING OF LOOPS USING IN SITU HI-C (5UM1HG009375-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9872199. Licensed CC0.

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