# SampleStream: A Radically Simplified Platform for Protein Sample Preparation

> **NIH NIH R44** · INTEGRATED PROTEIN TECHNOLOGIES, INC. · 2020 · $321,321

## Abstract

Project Summary:
Integrated Protein Technologies, Inc. is developing an analytical platform for the processing and analysis of
proteins via mass spectrometry. Our goal is to provide a highly flexible and scalable technology that offers unique
advantages in comparison to existing approaches, ultimately enabling new workflows while also leading to
significant cost reductions for existing proteomics experiments. During our Phase I award period, the technology,
the SampleStream™ platform, has undergone a major transformation, demonstrating proof-of-concept on all
proposed workflows. In essence, the fluidic device consists of a channel with an integrated molecular weight
cutoff membrane to which various flows are delivered. Protein can be stored in the channel for an extended
period of time, enabling chemical reactions as well as buffer exchange. Fluidic focusing is utilized to create a
narrow band of protein that can then be eluted from the channel in a manner similar to chromatography. The
workflows demonstrated during Phase I include rapid buffer exchange, protein reduction and denaturation, and
protein concentration, all carried out within the device. Furthermore, the device produced superlative analytical
performance in comparison to proposed milestones, yielding solid metrics of analytical performance. In the short
term, IPT has targeted biopharma as our initial beachhead market. With the advent, introduction, and rapid
growth of biologics, pharma companies are faced with a new and challenging array of analytical and automation
challenges. SampleStream technology provides a unique and streamlined method of sample preparation that
“shrink-wraps” complicated workflows into methods easily executed by technicians. While the SampleStream
platform will have a profound impact on the analysis of antibody- and other protein-based drugs, its application
space extends well beyond this initial market. IPT’s mission is to develop, manufacture and sell proteomics tools
that enable non-expert practitioners worldwide to generate proteomics data in a cost-effective manner across
many applications, including biopharma, medical diagnostics and basic research. To accomplish these aims,
IPT leverages its core competency in the analysis of intact protein molecules to create technologies that reduce
the cost and complexity of proteomics data acquisition. Ultimately, IPT believes that the commoditization of
proteomics measurements will have profound and transformative effects on everything from basic science to
personalized medicine.

## Key facts

- **NIH application ID:** 9873977
- **Project number:** 5R44GM121130-03
- **Recipient organization:** INTEGRATED PROTEIN TECHNOLOGIES, INC.
- **Principal Investigator:** Philip Daniel Compton
- **Activity code:** R44 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $321,321
- **Award type:** 5
- **Project period:** 2017-09-14 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9873977

## Citation

> US National Institutes of Health, RePORTER application 9873977, SampleStream: A Radically Simplified Platform for Protein Sample Preparation (5R44GM121130-03). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9873977. Licensed CC0.

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