# Aptamer Proteomics of Cardiometabolic and Renal Traits in African Americans

> **NIH NIH R01** · BETH ISRAEL DEACONESS MEDICAL CENTER · 2020 · $721,062

## Abstract

There is a disproportionate burden of cardiometabolic disease and chronic kidney disease (CKD) among
African Americans (AA), but the responsible environmental and/or genetic mechanisms are incompletely
defined. Quantitation of selected plasma proteins is clinically valuable, and new findings presented here argue
strongly that broadening the array of proteins analyzed can provide new insight into pathogenesis and offer
novel markers of disease risk and events. To address important knowledge gaps, we propose to analyze an
unprecedented array of proteins in plasma samples of AA in the Jackson Heart Study (JHS) and the Southern
Community Cohort Study (SCCS). We will test the overall hypothesis that proteomic profiling in well-
phenotyped cohorts will identify new pathogenic pathways of particular importance in AA.
In Specific Aim 1, we propose cross-sectional studies to identify novel proteomic biomarkers of cardiometabolic
and renal traits. In Specific Aim 2, we will assess whether baseline plasma protein concentrations predict
incident coronary heart disease (CHD) and CKD. For Specific Aims 1 and 2 the JHS will be the discovery
cohort; cross-sectional and longitudinal findings will be clinically validated in the SCCS. In Specific Aim 3, we
will use GWAS, exome sequence, and whole genome sequence to explore the genetic determinants of
proteins that are associated with cardiometabolic disease and CKD, and will test whether variants that are
associated with protein levels predict incident CHD and CKD in large meta-analyses.
We will leverage a powerful new analytic platform in which single-stranded DNA molecules called aptamers
have been modified to have high avidity and specificity for an array of 1,310 targeted plasma proteins. The
aptamers bind in proportion to each protein's concentration, and are released and quantified by fluorescence,
using microarrays. We present extensive experimental validation of these analyses including orthogonal
validation by liquid chromatography-mass spectrometry. Our multi-disciplinary team at the JHS, BIDMC, Broad
Institute, and Vanderbilt University brings expertise in proteomics, biomarkers, genetic and population
epidemiology, bioinformatics, and health disparities. All data will be made publically available, providing a
unique and valuable resource to the scientific community.

## Key facts

- **NIH application ID:** 9874009
- **Project number:** 5R01HL133870-05
- **Recipient organization:** BETH ISRAEL DEACONESS MEDICAL CENTER
- **Principal Investigator:** ROBERT E GERSZTEN
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $721,062
- **Award type:** 5
- **Project period:** 2017-04-01 → 2023-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9874009

## Citation

> US National Institutes of Health, RePORTER application 9874009, Aptamer Proteomics of Cardiometabolic and Renal Traits in African Americans (5R01HL133870-05). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9874009. Licensed CC0.

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