# Mayo Clinic Physical Activity Research Center: Metabolomics and Proteomics Analysis Site

> **NIH NIH U24** · MAYO CLINIC ROCHESTER · 2020 · $1,890,560

## Abstract

PROJECT SUMMARY
This proposal unites complementary analytical expertise of Mayo Clinic and University of Wisconsin in pursuit
of the goal of charting a molecular map of physical activity (PA). The overall goal of the Mayo Clinic Center for
Metabolomics and Proteomics (MCCMAPS) is to apply state-of-the-art metabolomics and proteomics
approaches to the MoTrPAC samples. We propose a metabolomics workflow that begins with untargeted
profiling to identify candidate metabolites. From there, annotated metabolites will be validated, and unknown
metabolites will be identified through a combination of novel compound synthesis, MS/MS data and NMR
spectroscopy structural elucidation. The important metabolites that emerge from untargeted profiling will guide
subsequent targeted quantitative metabolite measurements in plasma and tissue samples. We propose a
proteomics workflow that includes global quantitative proteomics in muscle, adipose, and exosomes and
assessment of posttranslational modifications using phosphoproteomics, acetylproteomics, and indices of
oxidative modifications. Global, discovery proteomics will guide targeted, quantitative proteomics of salient
proteins in plasma (e.g., irisin, myostatin). Our peptide synthesis capabilities allows for custom synthesis of
any stable isotope labeled peptides for standards for quantitation. In addition, we will measure stable isotope
enrichment of labeled amino acids in plasma, muscle compartments, and individual skeletal muscle proteins
thus offering an insight on how PA influences amino acid kinetics, protein metabolism, and translation of gene
transcripts to individual proteins. Isotopic enrichment of lipids in plasma and tissues will enable measurements
of fatty acid kinetics in response to PA. Advanced statistical methods will fuse proteomics and metabolomics
data to generate molecular concept maps of PA. Here we propose to leverage a productive ongoing
collaboration between metabolomics and proteomics experts at Mayo and University of Wisconsin as a single
integrated analytical resource dedicated to uncovering molecular signatures of PA. Our goal is to not only
contribute our analytical expertise, but also contribute our knowledge of muscle and adipose tissue biology to
the Molecular Transducers of Physical Activity Consortium (MoTrPAC).

## Key facts

- **NIH application ID:** 9878844
- **Project number:** 5U24DK112326-04
- **Recipient organization:** MAYO CLINIC ROCHESTER
- **Principal Investigator:** IAN R LANZA
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $1,890,560
- **Award type:** 5
- **Project period:** 2016-12-08 → 2022-11-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9878844

## Citation

> US National Institutes of Health, RePORTER application 9878844, Mayo Clinic Physical Activity Research Center: Metabolomics and Proteomics Analysis Site (5U24DK112326-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9878844. Licensed CC0.

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