# Cell and Tissue Imaging Core

> **NIH NIH P30** · UNIVERSITY OF SOUTHERN CALIFORNIA · 2020 · $98,642

## Abstract

ABSTACT
The Cell and Tissue Imaging Core (CTI) was created in 2007 in response to the evolving-growing needs of
RCLD Members. During the previous project period, the Microscopy Subcore provided fluorescence imaging
services within the CTI Core as one of two Subcores, with Histology as the other. Now, to enhance the
advanced imaging support for Center Membership, the CTI Core will serve as a stand-alone Core, entirely
focused on fluorescence imaging techniques. The Core will offer assisted imaging using confocal fluorescence
microscopy, with its Zeiss LSM 510 system. The Core has also expanded imaging services through campus
partnerships to enable 3D-structured illumination microscopy (3D-SIM), spinning disk confocal fluorescence
microscopy and multiphoton fluorescence microscopy in vivo for our Members. The Core's Specific Aims are:
1) To maintain the confocal and fluorescence microscopy facilities of RCLD. The Core will maintain facilities
according to rigorous standards of microscopy usage and image processing. 2) To provide assisted confocal
and fluorescence microscopy and image processing to Center Members. The Core will assist Center Members
to maximally utilize its own facilities in an efficient manner that enables them to achieve their research and
experimental goals. It will also aid them in state-of-the-art image analysis and preparation of publication quality
images. 3) To provide education and training in diverse microscopy applications supported by the Core. The
Core will provide theoretical and practical seminars and workshops focused in areas of microscopy supported
by the Core, including different modes of confocal fluorescence microscopy, fluorescence recovery after
photobleaching (FRAP), fluorescence resonance energy transfer (FRET), multiphoton fluorescence
microscopy and 3D-SIM. It will also provide smaller customized workshops, hands-on-training and individual
consultations for our Membership in these areas. 4) To provide access and financial support for RCLD Center
Members for the use of USC Core imaging facilities enabling 3D-SIM, spinning disk confocal microscopy and
multiphoton microscopy. While these instruments are available to all USC faculty on a fee-for-service basis
through the respective university Cores; the School of Medicine has generously provided a $50,000 annual
subsidy to Liver Center Members only to enable their access. Funds for access to these additional instruments
will be disbursed through quarterly submission of a brief pilot grant application for a maximum of $2,500
(renewal upon resubmission of a progress report) to the CTI internal advisory committee (CTI IAC) comprised
jointly of senior liver researchers and imaging specialists with expertise in the respective instruments.
Successful completion of these Aims will enable the utilization of state-of-the-art imaging advancing the
scientific progress of the RCLD membership.

## Key facts

- **NIH application ID:** 9883015
- **Project number:** 5P30DK048522-25
- **Recipient organization:** UNIVERSITY OF SOUTHERN CALIFORNIA
- **Principal Investigator:** Sarah F Hamm-Alvarez
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $98,642
- **Award type:** 5
- **Project period:** — → 2022-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9883015

## Citation

> US National Institutes of Health, RePORTER application 9883015, Cell and Tissue Imaging Core (5P30DK048522-25). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9883015. Licensed CC0.

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