# Cell Separation and Culture Core

> **NIH NIH P30** · UNIVERSITY OF SOUTHERN CALIFORNIA · 2020 · $169,659

## Abstract

ABSTRACT
The goal of the Cell Separation and Culture Core is to provide services that are frequently utilized by numerous
funded investigators so that we may share resources and reduce costs. The core facility also provides the
center members with an environment to promote scientific collaboration. In this application, we will continue
the services from the Cell Separation and Culture Core for hepatocyte isolation and culturing and liver and
immune cell sorting. In addition, we plan to expand our service by developing new techniques to meet the
growing demand of the center members, such as establishing hepatocyte cell lines stably expressing Cas9 to
enable the use of CASPR gene deletion system as well as isolation of liver progenitors and culturing of iPS
cells to support the ever growing field of liver stem cells. To accomplish these goals, we have the following
aims: 1) We will continue to provide high quality, consistent services needed by a large number of
independently funded investigators to improve research efficiency by sharing resources and lowering costs. 2)
We will foster an environment for interdisciplinary collaboration and growth. 3) We will provide training for
investigators at USC and elsewhere in techniques for cell isolation and separation. 4) We will develop new
services that the funded investigators and junior faculty will likely use in the future to advance their research in
the diagnosis, prevention and treatment of livers diseases.

## Key facts

- **NIH application ID:** 9883017
- **Project number:** 5P30DK048522-25
- **Recipient organization:** UNIVERSITY OF SOUTHERN CALIFORNIA
- **Principal Investigator:** Bangyan Stiles
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $169,659
- **Award type:** 5
- **Project period:** — → 2022-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9883017

## Citation

> US National Institutes of Health, RePORTER application 9883017, Cell Separation and Culture Core (5P30DK048522-25). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9883017. Licensed CC0.

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