# Clickable Microgel Scaffolds for MSC Expansion and Delivery

> **NIH NIH R01** · UNIVERSITY OF COLORADO · 2020 · $560,826

## Abstract

PROJECT SUMMARY
Repair of craniofacial bone defects is an important clinical problem with significant socioeconomic impact. Bone that is
traumatically injured or diseased often requires surgical repair, but 5-10% of bone fractures fail to heal and failure rates can
be even higher when the patient's bone quality is compromised (e.g., osteoporotic). In these cases, stem cell-based therapies
have received increasing attention as a method to improve the healing of complex craniofacial defects. The proposed
research focuses on mesenchymal stem cell (MSC) therapies because of their extensive use in clinical trials, as well as the
major role that MSCs play in musculoskeletal tissue homeostasis and the pathophysiology of osteoporosis. However, in
vitro expansion of MSCs to therapeutically relevant numbers reduces their regenerative capacity, and afterwards, direct
injection of MSCs alone often leads to low survival. The proposed research addresses this important clinical problem
through an innovative materials-based strategy, namely the synthesis and assembly of tunable microgel scaffolds for MSC
expansion and delivery. Using efficient “click” chemistries and by developing photoresponsive materials, we hypothesize
that scaffolds can be tuned to: i) prolong the self-renewing and regenerative capacity of MSCs during in vitro expansion
and ii) promote the survival and regenerative functions of delivered MSCs that will improve healing of both healthy and
osteoporotic bone. Specifically, we propose to: Aim 1. Develop a hydrogel culture system for MSC expansion and quantify
the effects of mechanical cues and passaging history on MSC proliferation, multipotency, secretory properties, and
epigenetic landscape; Aim 2. Process the hydrogel materials into modular microgel units for MSC delivery and tailor their
properties to promote MSC survival, retention and regenerative potential; and Aim 3. Test the influence of MSC expansion
conditions and modular microgel delivery systems on MSC survival and bone regeneration in vivo. If successful, this project
will have an important impact on public health by providing a powerful new platform for the expansion and site specific
delivery of MSCs. Given the versatility of the approach, which can be applied to numerous cell delivery systems, the results
will have broader implications that can extend beyond bone regeneration.

## Key facts

- **NIH application ID:** 9884753
- **Project number:** 5R01DE016523-12
- **Recipient organization:** UNIVERSITY OF COLORADO
- **Principal Investigator:** KRISTI S. ANSETH
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $560,826
- **Award type:** 5
- **Project period:** 2019-03-05 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9884753

## Citation

> US National Institutes of Health, RePORTER application 9884753, Clickable Microgel Scaffolds for MSC Expansion and Delivery (5R01DE016523-12). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9884753. Licensed CC0.

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