# Cholesterol Regulation of Lysosomes

> **NIH NIH R01** · STANFORD UNIVERSITY · 2020 · $392,500

## Abstract

Project Summary
The long term goal of this research is to understand how cholesterol gains
access to intracellular compartments for utilization, storage and cellular
regulation, via two glycoproteins: NPC1 and LAMP proteins. NPC1 is needed for
the egress of endocytosed, LDL-derived cholesterol from lysosomes into the
cytoplasm. LAMP proteins constitute the major glycoproteins that line the limiting
membranes of lysosomes. The goal of this application is to study how these
proteins may cooperate to transport cholesterol out of lysosomes, using
biochemical and cell biological approaches. Experiments are proposed to
investigate further, the cholesterol-dependent interaction between these proteins,
and how they, and their interactions, may influence downstream activation of
lysosome biogenesis. These experiments will provide important information
regarding how cells may sense and signal the availability of cholesterol in
lysosomes, and export cholesterol from lysosomes. This work has important
implications for a number of disease states including cardiovascular disease,
cancer, and neurological disorders.

## Key facts

- **NIH application ID:** 9888407
- **Project number:** 5R01HL134991-04
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** Suzanne R Pfeffer
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $392,500
- **Award type:** 5
- **Project period:** 2017-06-01 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9888407

## Citation

> US National Institutes of Health, RePORTER application 9888407, Cholesterol Regulation of Lysosomes (5R01HL134991-04). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9888407. Licensed CC0.

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