# Advanced Imaging Core

> **NIH NIH P30** · MEDICAL UNIVERSITY OF SOUTH CAROLINA · 2020 · $210,068

## Abstract

Advanced Imaging Core - Abstract
The overall goal of the Advanced Imaging Core is to provide DDRCC investigators with sophisticated
technologies, methods and training required for successful, high-end cell and tissue-based imaging and analysis,
including confocal, multiphoton and super-resolution microscopy, intravital imaging, and automated imaging of
histological slides. Specific Aims are: 1) Provide DDRCC investigators with state-of-the-art capabilities in optical
microscopy; 2) Develop novel, front-line imaging applications that address the emerging scientific needs of
DDRCC members; and 3) Provide expert mentoring, education, training, and consultation on sophisticated
optical imaging technologies that enable scientific discovery by DDRCC investigators, as well as the greater
MUSC research community. The Core houses the following major microscope systems: 1) Zeiss LSM 880 NLO
multiphoton/confocal system equipped with Quasar spectral detection and Fast Airyscan super-resolution
capability; 2) Olympus Fluoview FV1200 multiphoton microscope with silicone oil optics for intravital imaging; 3)
Olympus Fluoview FV 10i LIV live cell confocal microscope with water immersion optics; 4) Zeiss LSM 510 META
confocal microscope; 5) BD BioSciences CARV II disk-scanning confocal microscope for video-rate imaging; 6)
Zeiss Axiovert 200M widefield fluorescence microscope; and 7) Perkin-Elmer Vectra Polaris Automated
Quantitative Pathology Imaging System. Except for Vectra Polaris which is customized for histological slides, all
microscopes are equipped with environmental chambers for temperature and gas phase control to allow non-
destructive 3D imaging of living cells, tissues and organisms. Major applications include 1) live cell super-
resolution imaging of parameter-indicating fluorophores to monitor ions, electrical potentials, radical generation,
pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis) and the submicron
distribution of fluorescent proteins and other fluorescent reporters; 2) high resolution imaging of tissue sections
for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET)
and DuoLink to characterize and quantify interactions between specific molecules; 4) intravital microscopy to
monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, radical generation,
gene expression and other parameters in living animals; and 5) high throughput, quantitative multiplexed imaging
of conventionally and immuno-stained clinical and research specimens. Computer workstations provide offline
image processing/analysis (Bitstream Imaris, ImageJ FIJI, Metamorph, IPLab and others). Hands-on and
didactic training in current and next-generation imaging approaches are provided by seminars, demos and a
biennial 1-week Charleston Workshop on Light Microscopy for the Biosciences. The Advanced Imaging Core
services promote the success of D...

## Key facts

- **NIH application ID:** 9889236
- **Project number:** 1P30DK123704-01
- **Recipient organization:** MEDICAL UNIVERSITY OF SOUTH CAROLINA
- **Principal Investigator:** John J Lemasters
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $210,068
- **Award type:** 1
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9889236

## Citation

> US National Institutes of Health, RePORTER application 9889236, Advanced Imaging Core (1P30DK123704-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9889236. Licensed CC0.

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