# Exposure to violence, epigenetic variation, and asthma in Puerto Rican children

> **NIH NIH R01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2020 · $355,485

## Abstract

Puerto Rican children share a disproportionate burden from asthma in the U.S. We have demonstrated that in
Puerto Rican children, a variety of psychological stressors -including physical or sexual abuse, parental
psychopathology, and exposure to violence- are associated with asthma and worse asthma outcomes. In
particular, we have shown that exposure to violence is associated with childhood asthma in Puerto Ricans.
Moreover, we have reported that exposure to violence is associated with DNA methylation of the gene for the
pituitary adenylate cyclase activating polypeptide 1 type 1 receptor (ADCYAP1R1), and that such methylation
and a genetic variant in ADCYAP1R1 are each associated with asthma in Puerto Rican children. While
exposure to violence has been implicated in asthma and increased morbidity from asthma in children, we have
very limited knowledge about the epigenetic mechanisms underlying this link. Lack of such knowledge is an
important problem, because, without it, gaining the ability to prevent or treat asthma in underserved children
exposed to violence is highly unlikely. On the basis of our novel preliminary findings, we hypothesize that
exposure to violence increases the risk of asthma and asthma morbidity through altered methylation of genes
regulating behavioral, autonomic, neuroendocrine and immunologic responses to stress. To test this hypothesis,
we will first test for association between exposure to violence and genome-wide DNA methylation changes in
respiratory (nasal) epithelium in 500 Puerto Rican children (Sp. Aim 1). Next, we will examine whether
methylation changes in the top genes identified in Aim 1 are associated with asthma and measures of worse
asthma severity or control (severe asthma exacerbations, reduced lung function, and reduced bronchodilator
response) in 250 to 500 Puerto Rican children (Sp. Aim 2). Finally, we will assess the effects of methylation
changes in the genes identified in Aim 2 on gene expression in nasal epithelium of Puerto Rican children (Sp.
Aim 3a), as well as on gene expression and protein abundance in primary human bronchial epithelial cells (Sp.
Aim 3b). This proposal should determine how exposure to violence leads to increased risk of asthma and
asthma morbidity in underserved minority children, such as Puerto Ricans. To achieve this goal, we have
assembled an outstanding multidisciplinary research team.

## Key facts

- **NIH application ID:** 9889996
- **Project number:** 5R01MD011764-04
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Juan Carlos Celedon
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $355,485
- **Award type:** 5
- **Project period:** 2017-08-02 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9889996

## Citation

> US National Institutes of Health, RePORTER application 9889996, Exposure to violence, epigenetic variation, and asthma in Puerto Rican children (5R01MD011764-04). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/9889996. Licensed CC0.

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