# FGF signaling in lacrimal gland homeostasis, regeneration and disease

> **NIH NIH R01** · SCRIPPS RESEARCH INSTITUTE, THE · 2020 · $468,214

## Abstract

Summary
 This proposal investigates the fundamental molecular and cellular mechanisms of the fibroblast
growth factor (FGF) signaling that controls lacrimal gland (LG) stem and progenitor cell function and LG
growth and regeneration. These mechanisms are little studied and poorly understood. Defining factors that
can maintain stem/progenitor cell function in the damaged/inflamed LG will help us develop effective
therapies to treat LG dysfunction due to aqueous deficiency dry eye (ADDE). Our studies and recent
publications suggest that chronic inflammation impairs the epithelial stem cell niche and therefore
epithelial stem cell function [1-3]. Although many researchers (including our group) are adopting the
strategy of stem cell transplantation for tissue repair, an alternative approach to this therapy is to
manipulate the stem cell microenvironment, or niche, to facilitate LG repair by endogenous stem cells.
Through clonal cell-fate mapping studies using a multicolor Cre reporter combined with an in vivo
histone2B green fluorescent protein pulse chase strategy, we demonstrated for the first time, the
existence of multipotent LG epithelial stem cells in the basal layer of the duct as well as distinct long-lived
progenitor cells. Our preliminary studies describe the existence of novel epithelial niche supporting Fgf10-
expressing cells, able to maintain the survival and growth of stem/progenitor cells. These ductal FGF10-
expressing cells have a distinctive morphology and express the epithelial marker E-cadherin. Moreover,
they form an intimate interaction with the stem/progenitor cells within the basal cell layer of ducts. We
hypothesize that FGF-Fgfr2b signaling is required to maintain the epithelial stem and progenitor cell
populations.
 In the proposed research, we will determine specific roles for FGF10 expressing cells and FGF-Fgfr2b
signaling in lacrimal gland homeostasis and regeneration, will test the hypothesis that basal
stem/progenitor cells maintain and restrict their cell pool by generating the epithelial FGF10-expressing
niche-supporting cells, finally we will also test the therapeutic efficacy of FGF10 in resolving of LG
inflammation and improving the stem cell function.
 Our goal is to define the mechanisms/treatments able to reduce inflammation in chronically inflamed
LGs and protect stem/progenitor cells and therefore the function of the LG. The proposed studies will
better define the role of FGF signaling in LG homeostasis and inflammation and identify new therapeutic
strategies to improve the long-term health of LG and corneal surface.

## Key facts

- **NIH application ID:** 9891063
- **Project number:** 5R01EY028983-03
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Helen P. Makarenkova
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $468,214
- **Award type:** 5
- **Project period:** 2018-04-01 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9891063

## Citation

> US National Institutes of Health, RePORTER application 9891063, FGF signaling in lacrimal gland homeostasis, regeneration and disease (5R01EY028983-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9891063. Licensed CC0.

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