# Core B: Single cell and integrative genomics core

> **NIH NIH U19** · EMORY UNIVERSITY · 2020 · $316,549

## Abstract

The Single Cell and Integrative Genomics Core will provide support for individual Projects by performing
assays requiring specialized technology and offering unique bioinformatics methodology. More specifically,
Core B will be responsible for the following work: (i) conducting bulk and single-cell RNA-Seq profiling; (ii)
running bulk and single-cell ATAC-Seq assays to assess chromatin accessibility, and (iii) providing
computational expertise and bioinformatics for the analysis of Core B generated data that is beyond the
capabilities of individual Projects. Core B is led by Dr. Steven Bosinger at Emory University, with Dr. Will
Greenleaf at Stanford as a key Co-Investigator. Core B will be physically located at both Emory and Stanford.
The Emory site of Core B (Bosinger) will conduct bulk and single-cell RNA-Seq library preparation and
sequencing for the experiments described in each project. The Stanford site of Core B (Greenleaf) will be
responsible for ATAC-Seq library preparation on bulk and single-cell samples. Both sites will take advantage of
novel liquid handling platform work-flows to enable single-cell RNA-Seq and sc-ATAC-Seq library generation to
be performed in a high-throughput, cost-effective manner. Hence, Core B will be able to obtain information on
the epigenetic landscape of individual immune cells longitudinally after vaccination at a resolution and scale
that has previously not been feasible. Core B will also provide unique expertise in analyzing sc-ATAC-Seq data
that will take advantage of the availability of the sc-RNA-Seq data from matching samples. By integrating
single-cell RNA-Seq and ATAC-Seq data, these analytical approaches allow for epigenetic states that predict
transcriptional states to be identified with accuracy. Additionally, Core B, has developed methodology to
construct lymphocyte differentiation “trajectories” to order epigenetic and transcriptional changes at different
stages of development in single cells. This analytical approach will be applied to assess lymphocyte
differentiation following vaccination and in aging. In summary, the technological expertise offered by Core B
will allow for high-quality single-cell genomic data to be generated in a highly efficient and cost-effective
manner. More importantly, the integrated analytical pipelines developed and offered by Core B will be central
to attaining the research aims of each Project, specifically in building high resolution models of genetic states
that are imparted on lymphocytes during the acquisition of memory or senescence and identifying factors that
dictate these long-term fates.

## Key facts

- **NIH application ID:** 9893785
- **Project number:** 5U19AI057266-17
- **Recipient organization:** EMORY UNIVERSITY
- **Principal Investigator:** Steven Edward Bosinger
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $316,549
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9893785

## Citation

> US National Institutes of Health, RePORTER application 9893785, Core B: Single cell and integrative genomics core (5U19AI057266-17). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9893785. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*