# 3D Intestinal Tissues

> **NIH NIH U19** · TUFTS UNIVERSITY BOSTON · 2020 · $258,132

## Abstract

Core B – Summary
Core B will utilize the improvements developed for the 3D intestinal systems to support the preparation and
utility of the tissue models, including generating the scaffolds and seeded scaffolds/tissues for all projects. The
plans are based on our extensive preliminary data where we have demonstrated structurally- and functionally-
relevant 3D human intestinal tissues for the study of infectious diseases. The intestinal systems include
enteroid primary cell sources, a 3D porous protein scaffolding system with a geometrically-engineered hollow
lumen with adaptability to both large and small intestinal tissues. In Aim #1 the focus will be on the preparation
of scaffolds and 3D intestinal systems for the three research projects. Here the goal is to provide intestinal
tissues to support the three research projects (60 systems per week), obtain primary tissue samples from the
clinic, establish early passage organoid material from both the small intestine and the colon, and train
individuals in the various projects. In addition, a focus will be on documenting all materials for validation and
quality control to assure reproducibility. In Aim #2 the focus will be on optimization of the 3D intestinal tissues.
Here the goal is to continue to optimize the currently developed 3D human intestinal tissues generated from
organoids for physiological relevance, and develop increasingly complex features to facilitate analysis of
intestinal pathogens. These activities will include the incorporation of additional cell types, supplementation of
the scaffolds with ECM components, and further development of a bioreactor system to control flow, peristalsis
and oxygen levels. A team of faculty, post doctoral fellows and a technician will support the needs of Core B
and work closely with the research teams on all phases of the plans.

## Key facts

- **NIH application ID:** 9893797
- **Project number:** 5U19AI131126-04
- **Recipient organization:** TUFTS UNIVERSITY BOSTON
- **Principal Investigator:** DAVID L. KAPLAN
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $258,132
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9893797

## Citation

> US National Institutes of Health, RePORTER application 9893797, 3D Intestinal Tissues (5U19AI131126-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9893797. Licensed CC0.

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