# Regulation of eye development by an Opsin5-dopamine pathway

> **NIH NIH R01** · EMORY UNIVERSITY · 2020 · $463,580

## Abstract

ABSTRACT
 
Regulation of eye development by an Opsin 5-dopamine pathway. Neuropsin (OPN5) is an atypical
opsin that we show is expressed in retinal ganglion cells of the vertebrate retina (we call these ip5RGCs).
OPN5 is known to respond to near-UV wavelengths and to photoentrain a retinal circadian clock. Beyond
this, very little is known about the physiological function of neuropsin in any tissue.
 In this application we reveal that during development of the eye, an OPN5 response pathway
regulates levels of the crucial neuromodulator dopamine (DA). DA is known to have several functions in the
visual system but among them, the best characterized is its role in regulating the adaptive response to
nighttime and daytime lighting. We also reveal that DA has an important role directly influencing vascular
development within the eye. Preliminary data suggest that DA acts as a negative regulator of vascular
responses to promote hyaloid vessel regression and suppress retinal angiogenesis. Thus, DA acts as a
counter to VEGFA, a pro-vascular stimulus. Our preliminary findings suggest the central hypothesis: An
OPN5-dopamine pathway regulates vascular development of the eye. Our hypothesis incorporates the
dual suggestions that OPN5 regulates DA activity and that DA directly regulates vascular development in the
eye. We propose three experimental Aims designed to address the relationship between neuropsin activity
and DA production: Aim 1: To determine whether ip5RGCs form physical connections with dopaminergic
amacrine cells. Aim 2: To assess whether OPN5 regulates dopamine acutely or via the retinal circadian
clock. Aim 3: To determine the mechanism by which DA regulates vascular development in the eye. When
complete, these analyses will provide important new insights on the role of neuropsin in the physiology of the
visual system. This analysis is also clinically relevant because a DA-vascular pathway is probably the
explanation for the association between DA treatment of premature infants (for hypotension) and the
potentially blinding vascular overgrowth of severe retinopathy of prematurity.

## Key facts

- **NIH application ID:** 9893913
- **Project number:** 5R01EY027711-04
- **Recipient organization:** EMORY UNIVERSITY
- **Principal Investigator:** P Michael Iuvone
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $463,580
- **Award type:** 5
- **Project period:** 2017-04-01 → 2021-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9893913

## Citation

> US National Institutes of Health, RePORTER application 9893913, Regulation of eye development by an Opsin5-dopamine pathway (5R01EY027711-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9893913. Licensed CC0.

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