# The role of Arf tumor suppressor in translational reprogramming

> **NIH NIH F32** · WASHINGTON UNIVERSITY · 2020 · $65,310

## Abstract

ABSTRACT
The ARF tumor suppressor is frequently mutated or downregulated in cancer. The major role of ARF is to
activate p53 by sequestering the p53 regulator Mdm2. ARF also plays a significant role in ribosome biogenesis
and mRNA translation. Previously, loss of Arf has been shown to cause a bulk increase in ribosome
biogenesis, ribosome export into the cytoplasm and an increase in global protein synthesis. Activation of ARF
inhibits these processes. Notably, ARF is known to specifically regulate the translation of several mRNAs.
Based on previously published data we hypothesize that ARF regulates the translation of IRES containing
mRNAs through displacement of RpL11 from the ribosome. To address our hypothesis, we propose three
aims: 1) To map global translational regulation upon ARF loss or gain using ribosome profiling. 2)
To assess the regulation of IRES containing mRNAs by ARF. 3) To identify proteins involved in ARF-
dependent translational reprogramming

## Key facts

- **NIH application ID:** 9894641
- **Project number:** 5F32GM131514-02
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Kyle Cottrell
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $65,310
- **Award type:** 5
- **Project period:** 2019-03-01 → 2021-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9894641

## Citation

> US National Institutes of Health, RePORTER application 9894641, The role of Arf tumor suppressor in translational reprogramming (5F32GM131514-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9894641. Licensed CC0.

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