# High Resolution Imaging Shared Facility

> **NIH NIH P30** · UNIVERSITY OF ALABAMA AT BIRMINGHAM · 2020 · $170,282

## Abstract

IMAGING GROUP 
HIGH RESOLUTION IMAGING SHARED FACILITY (HRISF) 
ABSTRACT 
The detection and imaging of molecules in biological systems is a vitally important tool in cancer research. As 
the genetic basis for cancers are identified, the subsequent objectives are to identify the localization, functions, 
and interactions of gene products and to determine their role in the initiation and progression of disease. 
These objectives require highly sophisticated methodologies for the analysis of molecular events in vitro, in live 
cells, and in animal models of disease. Therefore, the High Resolution Imaging Shared Facility (HRISF) 
provides Comprehensive Cancer Center investigators with access to complex instrumentation required for the 
detailed microscopic analysis of cells and tissues and the expertise to use it productively. The imaging 
resources available include: 1) digital imaging; 2) Ca2+ imaging; 3) a FRET system; 4) a Zeiss LSM 710 
equipped for fluorescence lifetime imaging (FLIM); 5) a Nikon A1R high speed confocal system optimized for 
live cell and tissue imaging; and 6) a Nikon A1R Multiphoton system optimized for intravital imaging with a 
Coherent Chameleon Vision II ultrafast laser. In addition, the Nikon A1R Multiphoton system is mounted on a 
vibration isolation table and has all of the equipment required for long-term animal anesthesia, respiration, and 
body temperature maintenance. Recent additions to the shared resource include an ImageStream X Imaging 
cytometer which allows high throughput imaging - over 5000 cells/second can be imaged with up to 12 
images/cell. We also obtained a Nanosight NS300 which allows characterization of exosomes and 
nanoparticles. For ultrastructural studies the HRISF offers an FEI Quanta 650 FEG Environmental Scanning 
EM and a Tecnai T12 Spirit TWIN transmission electron microscope. Cancer-related research supported by 
the HRISF includes translational studies that range from fundamental studies of acquired resistance to frontline 
cancer therapeutics. The HRISF served over 88 CCC investigators during the current CCSG cycle and their 
use accounted for 49.5% of the total facility usage during this same period. At the same time, support from the 
CCSG accounted for 23% of the total annual operating costs of the shared resource.

## Key facts

- **NIH application ID:** 9895647
- **Project number:** 5P30CA013148-48
- **Recipient organization:** UNIVERSITY OF ALABAMA AT BIRMINGHAM
- **Principal Investigator:** Alexa Lynn Mattheyses
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $170,282
- **Award type:** 5
- **Project period:** — → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9895647

## Citation

> US National Institutes of Health, RePORTER application 9895647, High Resolution Imaging Shared Facility (5P30CA013148-48). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9895647. Licensed CC0.

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