# A conditional and rapid cell ablation technology in zebrafish

> **NIH NIH R03** · UNIVERSITY OF MISSOURI-COLUMBIA · 2020 · $74,926

## Abstract

PROJECT SUMMARY
Targeted cell ablation is a powerful approach for investigating cellular functions, tissue regeneration,
developmental processes and disease pathogenesis. Several genetic methods are available for cell ablation,
but the ablation process is relatively slow, requiring at least several hours. This can be a disadvantage when
examining relatively rapid cellular events (ranging from seconds to minutes) during development or in
physiology. This proposal tests the feasibility of a novel genetic cell ablation technology established in
mammals for mediating rapid cell ablation in zebrafish. Preliminary data indicate that the system works very
well in vitro with cultured zebrafish neurons. These studies will optimize the conditions for effective and rapid
cell ablation in vivo, in gastrulating embryos to swimming larvae.

## Key facts

- **NIH application ID:** 9896887
- **Project number:** 5R03HD098555-02
- **Recipient organization:** UNIVERSITY OF MISSOURI-COLUMBIA
- **Principal Investigator:** Anand Chandrasekhar
- **Activity code:** R03 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $74,926
- **Award type:** 5
- **Project period:** 2019-04-01 → 2023-09-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9896887

## Citation

> US National Institutes of Health, RePORTER application 9896887, A conditional and rapid cell ablation technology in zebrafish (5R03HD098555-02). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9896887. Licensed CC0.

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