# Cytometry Core

> **NIH NIH P01** · BOSTON CHILDREN'S HOSPITAL · 2020 · $201,338

## Abstract

Project Summary: Cytometry core
The key objective of the Cytometry Core is to enhance the overall productivity of the Program by providing a
cost-effective, centralized resource for the use of antibody based cellular pre-enrichment, subsequent flow
cytometry based analysis and purification, thin and whole/thick frozen sections for 2D and 3D imaging, and
quantitative imaging cytometry. The core will provide 1) the 8 parameter flow cytometer that is the workhorse
analytical instrument for the majority of flow cytometry experiments proposed in all three projects, 2) a
centralized, cost-effective resource for the laser scanning cytometry (LaSC) for quantitative imaging cytometry
analysis proposed in all three projects, 3) preparation of thick cryostat tissue sections and 3D confocal imaging, 4) a
resource for expert advice and consultation in the design and analysis of complex, multicolor flow cytometry
and LaSC experiments including FlowJo software licenses for flow cytometry analysis and iCys cytometric
analysis software (CompuCyte) for LaSC analysis, and 5) magnets and reagents for cellular rosetting and
magnetic-particle based strategies for pre-enrichment of cells of interest prior to subsequent flow cytometry
based analysis and/or sorting. By providing a cost-effective, centralized resource for antibody based cellular
pre-enrichment and subsequent cytometry (flow cytometry and LaSC) based analysis and purification, the
Cytometry core will help further the Programs goals of understanding the pathways which control the function
of transfused bone marrow, cord blood, and peripherally derived cells.

## Key facts

- **NIH application ID:** 9897589
- **Project number:** 5P01HL095489-10
- **Recipient organization:** BOSTON CHILDREN'S HOSPITAL
- **Principal Investigator:** Shin-Young Park
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $201,338
- **Award type:** 5
- **Project period:** — → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9897589

## Citation

> US National Institutes of Health, RePORTER application 9897589, Cytometry Core (5P01HL095489-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9897589. Licensed CC0.

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