# Cell cycle regulation in response to plasma membrane stress in S. cerevisae

> **NIH NIH SC1** · BROOKLYN COLLEGE · 2020 · $353,250

## Abstract

Project Summary/Abstract
 Yeast cells need to rapidly control their cell cycle profile, gene expression or protein
stability in response to the activation of stress response pathways. It is common for cells to
suffer from various attacks to their plasma membrane and cell wall, such as physical damage,
pathogen invasion and various environmental perturbations. Here we propose to study
that plasma membrane stress inhibits cell growth and DNA replication through a novel cell cycle
checkpoint pathway. Eukaryotic cells duplicate their chromosomes. Errors in the DNA
replication control could lead to chromosome instability which is a hallmark of cancer. Our long-
term goal is to understand the molecular mechanism of DNA replication control in response to
stress.
 In this proposal, three aims will test my hypothesis if plasma membrane damage
inhibits polarized cell growth and DNA replication through a novel cell cycle checkpoint
pathway.
Aim1 will determine how membrane damage drives Pkc1 relocation to inhibit polarized cell
growth.
Aim2 will determine how plasma membrane stress signal activates Mpk1 to inhibit DNA
replication.
Aim3 will determine if a yeast GSK-3 kinase Mck1 targets replication proteins upon plasma
membrane stress
DNA replication and membrane integrity pathways are well conserved from yeast to humans.
The results obtained from this study can be applied to understand the molecular mechanism of
DNA replication control in response to stress in higher eukaryotes.

## Key facts

- **NIH application ID:** 9897602
- **Project number:** 5SC1GM121242-04
- **Recipient organization:** BROOKLYN COLLEGE
- **Principal Investigator:** Amy E Ikui
- **Activity code:** SC1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $353,250
- **Award type:** 5
- **Project period:** 2017-04-01 → 2021-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9897602

## Citation

> US National Institutes of Health, RePORTER application 9897602, Cell cycle regulation in response to plasma membrane stress in S. cerevisae (5SC1GM121242-04). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9897602. Licensed CC0.

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