# Host Response Monitoring

> **NIH NIH U19** · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · 2020 · $217,266

## Abstract

Abstract 
The overall objective of Core C: Host Response Monitoring is to provide comprehensive and consistent 
immune monitoring to enhance the activities of the GV-CRC program. The center will advance promising 
candidate N. gonorrhea vaccines in a rigorous and systematic manner so that one or more candidates have a 
feasible path for licensure at the end of the five-year funding period. The center is focused on innovative 
vaccine platforms, immunogens and adjuvant formulations. This GV-CRC immunology-focused core will 
provide centralized assay platforms for detailed and comprehensive analysis of host immune response for all 
three GV-CRC projects. Core C is therefore essential to the overall success of the GV-CRC program to 
develop vaccines against N. gonorrhea, a major public health threat. To accomplish Core goals, the efforts 
and expertise of Dr. Sempowski's cellular immunology laboratory and Dr. Staats' mucosal immunology 
laboratory have been united. The Core will be run out of the NIAID-Regional Biocontainment Laboratory at 
Duke University in the Duke Human Vaccine Institute. Drs. Sempowski and Staats are experts in systemic and 
mucosal immune monitoring in small animals and human. In addition, Dr. Staats is a thought-leader in vaccine 
adjuvant discovery and development and will bring this expertise to the GV-CRC. Proposed centralized 
immune monitoring approaches have been divided into three specific aims. 1) Humoral Responses: Provide 
high-throughput antibody quantity and isotype assessment by ELISA, and antibody quality (avidity) by surface 
plasmon resonance; 2) Cellular Responses: Provide expertise in cellular subset isolation, polychromatic flow 
cytometry/cell sorting, and functional in vitro restimulation assays for comprehensive host response monitoring 
to both experimental vaccines/adjuvants and challenge pathogens; and 3) Multiplex Biomarker Analysis: 
Provide targeted multiplex array profiling of biological samples, such as culture supernatant, serum/plasma, 
urine or inflammatory exudate, using the luminex bead array platform. The Core will support all four GV-CRC 
research projects and Core leadership will work closely with the center PI and project leaders to ensure that 
the needs of all projects are rigorously met in a reliable, reproducible and timely manner.

## Key facts

- **NIH application ID:** 9900722
- **Project number:** 5U19AI144180-02
- **Recipient organization:** HENRY M. JACKSON FDN FOR THE ADV MIL/MED
- **Principal Investigator:** Andrew Macintyre
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $217,266
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9900722

## Citation

> US National Institutes of Health, RePORTER application 9900722, Host Response Monitoring (5U19AI144180-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9900722. Licensed CC0.

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