# Quantitative measurement of whole-body glycogen metabolism and gluconeogenic flux

> **NIH NIH F32** · PRINCETON UNIVERSITY · 2020 · $65,310

## Abstract

Project Summary/Abstract
Dysregulated endogenous glucose production is the central cause of type 2 diabetes. Recently published work
from the lab of the sponsor determined that lactate carries the highest circulatory turnover flux and is an
important TCA substrate. During these studies, a general observation made from intravenous infusion of 13C-
glucose in freely moving mice was surprisingly low labeling of glycolytic intermediates in all tissues in both fed
and fasted mice. What is the source of glycolytic intermediates if not circulating glucose? We have two
hypotheses: 1) glycolytic intermediates come from glycogen and 2) gluconeogenesis produces tissue glycolytic
intermediates. If either of these are true, these pathways are active more often and, in more tissues, than
previously thought. Here, we will utilize state of the art LC-MS metabolomics in combination with intravenous
infusion of 13C-metabolite tracers to measure these pathways in fed and fasted mice in a wide array of tissues.
LC-MS offers advantages when measuring flux through these pathways including the ability to 1) measure
labeling in glycolytic intermediates and 2) to determine quantitative labeling patterns. Utilizing this
methodology, we aim to gain a comprehensive understand of glycogen metabolism and gluconeogenesis in all
tissues. This work will not only deepen our understanding of these important metabolic pathways, but it has the
potential to uncover novel roles for these pathways in maintaining tissue and organismal health.

## Key facts

- **NIH application ID:** 9901357
- **Project number:** 5F32DK118856-02
- **Recipient organization:** PRINCETON UNIVERSITY
- **Principal Investigator:** Tara A. TeSlaa
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $65,310
- **Award type:** 5
- **Project period:** 2019-05-01 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9901357

## Citation

> US National Institutes of Health, RePORTER application 9901357, Quantitative measurement of whole-body glycogen metabolism and gluconeogenic flux (5F32DK118856-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9901357. Licensed CC0.

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