# Structure mapping of molecular complexes by super-resolution microscopy

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2020 · $342,834

## Abstract

PROJECT SUMMARY
Eluciding the structural organization of macromolecules or molecular complexes is one of the fundamental
steps towards mechanistic understanding of their function and activity regulation. With atomic level details
elucided by traditional structural biology techniques such as crystallography, nuclear magnetic resonance and
cryo-electron microscopy, the next step is to place this structural information in to the cellular context. For this
purpose, we take the approach of using super-resolution fluorescence microscopy to map the spatial
coordinates of their individual components. For this approach, we plan to develop a scalable method for
efficient alabeling endogenous proteins for super-resolution microscopy, as well as analysis algorithms for
super-resolution images. We will apply this approach to the study of basal body - cilium transition zone, which
has been shown in our preliminary study to be a gate structure that regulates protein trafficking in the cilium.

## Key facts

- **NIH application ID:** 9902489
- **Project number:** 5R01GM124334-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** Bo Huang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $342,834
- **Award type:** 5
- **Project period:** 2018-05-04 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9902489

## Citation

> US National Institutes of Health, RePORTER application 9902489, Structure mapping of molecular complexes by super-resolution microscopy (5R01GM124334-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9902489. Licensed CC0.

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