# Research Support Core: Genetics and Metabolomics

> **NIH NIH P42** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2020 · $264,903

## Abstract

Core Summary/Abstract
Studies of Superfund toxicants require novel mouse models, careful, high-throughput analyses of hormones,
cytokines and small molecule metabolites, and informatics analyses of large datasets to pioneer new findings
in the detection of environmental toxicants and health effects of exposure. The UC San Diego Superfund
Research Center Genetics and Metabolomics Core facility will provide state-of-the-art molecular biology
services for generation of murine models of toxicant exposure as well as cutting-edge analytical services for
sensitive, high-throughput assay of hormones and small molecule metabolites and their informatic analyses.
This Core will interface with all six proposed research projects. Comprehensive approaches for modifying the
mouse genome are provided including DNA microinjection, embryonic stem cell homologous recombination,
CRISPR/Cas9 mutagenesis, and blastocyst injection, specifically tailored to UCSD Superfund projects.
Furthermore, metabolomics services will be provided, allowing for measure of hormones, growth factors, and
thousands of small molecule metabolites (both targeted and untargeted) using a Luminex magnetic bead
analyzer and liquid chromatography-mass spectrometry based approaches. Samples for analysis will include
human and mouse biospecimens (plasma, urine, and stool) as well as community plant, water, and soil
samples. Bioinformatics services will provide in-depth analyses of large datasets resulting from
transcriptomics, cistromics, and metabolomics. This core provides centralized, cost-effective, efficient,
technically sophisticated services that are crucial to the success of all six projects in the UC San Diego
Superfund Research Center.

## Key facts

- **NIH application ID:** 9903334
- **Project number:** 5P42ES010337-19
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** PAMELA L MELLON
- **Activity code:** P42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $264,903
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9903334

## Citation

> US National Institutes of Health, RePORTER application 9903334, Research Support Core: Genetics and Metabolomics (5P42ES010337-19). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9903334. Licensed CC0.

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