# Single cell, multi-parametric high throughput platform to classify endocrine disruptor potential of mixtures

> **NIH NIH P42** · TEXAS A&M UNIVERSITY · 2020 · $212,685

## Abstract

Project 4 Abstract
Human health is at risk due to environmental exposures to a wide range of chemical toxicants and endocrine
disrupting chemicals (EDCs). EDCs are commonly found in natural and industrial sources and represent a
large and growing family of compounds and mixtures with high chemical diversity. The Texas A&M Superfund
Research Center is focused on evaluating and reducing the human health risks posed by exposure to
hazardous substances, including EDCs, that are mobilized from sediments and other sources by
environmental disaster events such as tropical storms, hurricanes, and floods. The overarching goal of
Project 4 is to develop fast, robust, and cost-effective assays to determine the endocrine disrupting potential of
complex, environmentally relevant chemical mixtures in the highly industrial Galveston Bay/Houston Ship
Channel area (GB/HSC) and other Superfund sites. The availability of a set of fast, sensitive, and reproducible
EDC assays will facilitate health hazard evaluation and improve decision-making in response to environmental
emergencies. The central hypothesis that will be tested in Project 4 is that multi-parametric, highly
mechanistic contextual in vitro assays, and bioinformatics analyses will serve as a robust, fast, and cost-
effective framework to evaluate the endocrine disrupting potential of environmentally-derived complex chemical
mixtures. Nuclear Receptors (NRs), a large class of transcription factors, are key mechanistic targets of EDCs.
The assays developed in Project 4 will focus on three NRs identified by the Endocrine Disruptor Screening and
Testing Advisory Committee: estrogen receptor (ER), androgen receptor (AR), and thyroid hormone receptor
(TR). We will use advanced high throughput imaging and image analysis, genome-wide epigenomics, and
integrative bioinformatics to address critical needs in assessing the risk to human health posed by hazardous
substances: 1) Development of fast, robust, and cost effective high throughput assays to identify the presence
of EDCs and classify their bioactivity. Cell-based platforms in endocrine-relevant systems will be used to
analyze both single compounds (~50, including heavy metals, pesticides, pharmaceuticals, etc.), and complex
mixtures, including samples from Superfund sites. 2) Comprehensive analysis of EDC impact on the
epigenome and the identification of EDC-specific epigenetic “fingerprints.” The epigenetic impact of EDCs has
not previously been considered as a way to identify and classify EDCs, and has the potential to provide new
insights into EDC- and NR-specific mechanisms of pathway disruption. 3) Assessment of the endocrine
disrupting potential of chemical mixtures in the environment. While previous work has primarily focused on
EDC activity of single compounds mediated by the ER, we will increase the scope and relevance of EDC
assessment by measuring effects on AR and TR, and by moving from single compounds to mixtures. High
content, cell-based, and ...

## Key facts

- **NIH application ID:** 9903372
- **Project number:** 5P42ES027704-04
- **Recipient organization:** TEXAS A&M UNIVERSITY
- **Principal Investigator:** MICHAEL A. MANCINI
- **Activity code:** P42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $212,685
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9903372

## Citation

> US National Institutes of Health, RePORTER application 9903372, Single cell, multi-parametric high throughput platform to classify endocrine disruptor potential of mixtures (5P42ES027704-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9903372. Licensed CC0.

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