# Recombinational biopanning method for multiplex antibody:antigen screening

> **NIH NIH R01** · ABCAM, INC. · 2020 · $557,891

## Abstract

ABSTRACT
There has been tremendous progress in DNA sequencing technology to decrease cost and
increase capacity, yet technologies for proteomics have not advanced much in the last 30 to 40
years. While a number of approaches for generating recombinant affinity reagents exist, the cost
and throughput of current technologies represent significant roadblocks to the development of a
comprehensive and broadly available resource of renewable affinity reagents. We propose to
develop a technology that will allow us to screen a library of M13 phage displaying >1010 different
antibodies against a library of >104 different protein (and/or peptide) antigens displayed on an E.
coli cell surface. The F-pilus is the attachment site for M13 infection of E. coli. In the proposed
technology, phage infection of the cells relies on the interaction of an M13-displayed antibody and
the bacterial cell surface-displayed antigen of a transiently-F-minus E. coli host in water-in-oil
emulsions. NextGeneration DNA sequencing and a multiplex barcoding scheme will be used to
identify antibody:antigen pairs. We will use a set of high-value, immuno-oncology targets to
develop the method and compare to antibodies obtained with more traditional phage display
biopanning techniques. The technology itself should be applicable to any organism, including
viral, bacterial and human proteomes, as well as single- and mixed-populations of proteomes.

## Key facts

- **NIH application ID:** 9904470
- **Project number:** 5R01AI135151-03
- **Recipient organization:** ABCAM, INC.
- **Principal Investigator:** MICHAEL P WEINER
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $557,891
- **Award type:** 5
- **Project period:** 2018-05-14 → 2021-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9904470

## Citation

> US National Institutes of Health, RePORTER application 9904470, Recombinational biopanning method for multiplex antibody:antigen screening (5R01AI135151-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9904470. Licensed CC0.

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