# Core H: Protein Expression and Proteomics

> **NIH NIH P30** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2020 · $222,525

## Abstract

Project Summary/Abstract
Core H - The purpose of the Protein Expression and Proteomics Analysis (PEP) Core is to provide state-of-
the-art technology, equipment and expertise for the cloning, expression and purification of recombinant
proteins required by the San Diego (SD) CFAR community, as well as the tools and expertise required for
molecular analysis of proteins and protein mixtures. In particular, the PEP Core will provide protein expression
and analytical tools not available to most laboratories and at a reduced cost affordable for CFAR members.
The PEP Core is divided into two units based on services provided:
The Protein Expression Unit (Co-Director, John Elder) will aid CFAR members as needed for cloning,
expression and/or purification of proteins, based on the needs of the investigator. This unit will also maintain
stocks of frequently requested viral and immunological proteins such as HIV gp120 and Gag antigens and will
aid in the purification of antibodies, as required.
The Proteomics Unit (Co-Director, Bruce Torbett) maintains state-of-the-art equipment and capabilities for
protein analysis including nanospray mass spectrometer, HPLC, Typhoon 3-color laser for analysis of protein
mixtures, light cycler instrumentation for analyzing protein mixtures, and equipment for 1- and 2-dimensional
protein separations. These technologies and provided expertise will aid investigators in quantitative and
qualitative analysis of their proteins and protein mixtures from cell samples.
In addition, the PEP Core will provide training to CFAR members in all facets of protein expression and
analysis offered by the Core, as requested by the community. Information will be provided by both one-on-one
interactions, as well as through seminars on various techniques, based on the interests of the members. The
long-term goal of the Core is to help facilitate efficient and economical analysis of proteins required by the
CFAR membership to aid in the more rapid development of an understanding of the HIV life cycle and the
development of intervention strategies against the global spread of HIV.

## Key facts

- **NIH application ID:** 9906064
- **Project number:** 5P30AI036214-26
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Bruce Edward Torbett
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $222,525
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9906064

## Citation

> US National Institutes of Health, RePORTER application 9906064, Core H: Protein Expression and Proteomics (5P30AI036214-26). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/9906064. Licensed CC0.

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