# Renal Oxygenation and Mitochondrial Function in AKI

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2020 · $348,750

## Abstract

PROJECT SUMMARY
Acute kidney injury (AKI) in the setting of sepsis is frequently observed and is a significant
clinical problem with high levels of morbidity and mortality. One of the major barriers to the
progress in the field is the lack of understanding of the pathogenesis of AKI in this setting.
The specific aims of this proposal is to investigate the primary and/or secondary changes in
mitochondrial function and morphology in sepsis associated AKI that lead to inefficient oxygen
utilization by the kidney. The proposal also aims to determine the effects of mitochondrial
dysfunction and elevated oxygen utilization in the kidney on overall renal function and kidney
injury in the setting of sepsis.
The research strategy is to employ a comprehensive investigative approach for an integrative
understanding of pathogenesis of sepsis associated AKI, using the clinically relevant cecal
ligation and puncture model of sepsis. The methods will include physiological techniques such
as whole animal kidney clearance, renal blood flow and micropuncture and molecular
techniques to assess mitochondrial bioenergetics, ATP, reactive oxygen species generation and
electron microscopy to assess mitochondrial morphology and dynamics. These investigations
will provide important insights into hemodynamic and non-hemodynamic factors in the
pathogenesis of sepsis-associated AKI and identify specific mechanistic pathways and novel
therapeutic targets. The insights obtained will be will be valuable beyond the model studied
given the wide-spread implications of mitochondrial dysfunction in kidney disease.

## Key facts

- **NIH application ID:** 9906221
- **Project number:** 5R01DK107852-05
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Prabhleen Singh
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $348,750
- **Award type:** 5
- **Project period:** 2016-08-18 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9906221

## Citation

> US National Institutes of Health, RePORTER application 9906221, Renal Oxygenation and Mitochondrial Function in AKI (5R01DK107852-05). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9906221. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*