# New Generation Blood Exchange Devices for Enhancing Tissue Regeneration and Health

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA BERKELEY · 2020 · $385,400

## Abstract

Exchange transfusion (apheresis) is a routine strategy for the management of several diseases, such as
sickle cell disease, hemolytic disease of newborns, autoimmune disorders, etc. Interestingly, the body of
published work on heterochronic parabiosis, the surgical joining of two animals of different ages, and more
recently, our study on heterochronic blood exchange, suggest that blood apheresis can be repositioned and
used as a new modality: restoring the circulatory environment of aged mammals back to a productive, young,
composition, may help to rapidly and broadly enhance the maintenance and repair of multiple organs,
combatting a number of degenerative diseases of brain, muscle, liver, etc. and inflammatory disorders.
 In contrast to the permanent anastomosis of parabiosis, in our small animal blood exchange system
animals are connected and disconnected at will, removing the influence of shared organs, adaptation to being
joined, etc. Unlike parabiosis, where joint circulation is stochastically established in ~7-10 days, our procedure
is less invasive and accurately controlled, the exchange volumes are easily programmed and measured; and
the onset and duration of the effects can be accurately and with ease interrogated. The cells versus plasma
heterochronicity can be studied and immuno-affinity modules enable removal of specific inhibitory molecules
from the old circulation prior to return of such “rejuvenated” blood into the old animal; as well as a screen for
candidate systemic proteins, removal of which from young circulation inhibits tissue maintenance and repair.
 We will (1) determine the onset and duration of the effects of heterochronic blood apheresis on health and
maintenance of muscle, liver and brain, animal performance (strength and cognition), and systemic cytokine
profile, also defining the minimal functional dose of exchanged blood that significantly influence these
parameters; (2) engineer the V2 device where, in continuous apheresis between two animals of different age,
circulating leukocytes are separated from plasma, studying how a cross-match of the age of cells and plasma
influences tissue maintenance and repair; (3) engineer the V3 device with imunoaffinity modules allowing to
screen and attenuate to young – healthy levels candidate systemic proteins, using IL6 and TNF-a as the first
candidates, that when elevated with age have negative effects on tissue health and regeneration.

## Key facts

- **NIH application ID:** 9906269
- **Project number:** 5R01HL139605-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA BERKELEY
- **Principal Investigator:** Kiana Aran
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $385,400
- **Award type:** 5
- **Project period:** 2018-04-21 → 2022-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9906269

## Citation

> US National Institutes of Health, RePORTER application 9906269, New Generation Blood Exchange Devices for Enhancing Tissue Regeneration and Health (5R01HL139605-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9906269. Licensed CC0.

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