# Integrating functional genomics in primary human adipocytes to investigate gene regulatory circuitry for obesogenic cardiovascular traits

> **NIH NIH F31** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2020 · $37,098

## Abstract

ABSTRACT
Abnormal serum lipid levels, or dyslipidemias, are risk factors for cardiovascular disease (CVD), the leading
cause of death worldwide. The genetic factors underlying serum triglyceride (TG) levels are not well
understood, despite this trait exhibiting ~50% heritability. Adipose tissue is an important endocrine organ for
lipid homeostasis, and adipocytes are key players in energy intake and expenditure mediated through free fatty
acid uptake and TG storage and mobilization. The goal of this project is to improve the current understanding
of genomic regulatory mechanisms in gene expression and lipid processing pathways in adipocytes. Aim 1 is
targeted to identify serum TG-correlated, adipose-expressed genes that are under local genetic control by
expression quantitative trait loci (cis-eQTLs) in the Finnish METabolic Syndrome In Men (METSIM) cohort. We
will use promoter Capture Hi-C (pCHi-C) in primary human white adipocytes (HWA) to identify which adipose
cis-eQTLs interact with the target gene promoter, as promoter-interacting regions are enriched for regulatory
elements. Our preliminary data support this, showing that SNPs in open chromatin within promoter-interacting
regions in HWA contribute significantly to the heritability of adipose gene expression and serum TG levels in
the METSIM cohort. By further integrating lipid genome-wide association study (GWAS) loci into our analysis,
we can find a target gene and underlying mechanism of the GWAS signal. In Aim 2 we will perform RNA-
sequencing, pCHi-C, and Assay for Transposase-Accessible Chromatin (ATAC)-seq in primary HWA after
treating them with saturated or monounsaturated fatty acids, to investigate the genomic regulatory
mechanisms altered in response to lipid challenge. This will facilitate detection of elements mediating gene
expression changes, assayed through both pCHi-C and ATAC-seq. We hypothesize that genomic regulatory
architecture uncovered in this system can be used across populations. We will thus use our data to examine
Mexican-specific regions found to be associated with high serum TGs in our laboratory. One locus on
chromosome 11 contains a risk haplotype for both high TGs and increased post-prandial TG levels after a fatty
meal. The regulatory circuitry, identified in HWA both before and after lipid challenge, can be highly valuable
for understanding how Mexican-specific variation might lead to genetic dysregulation at this locus. Aim 2 will
also use the UK Biobank (UKBB) for genotype-by-environment interaction analyses. Many systemic metabolic
disturbances that are more likely to be present in obese and overweight individuals involve adipocyte function.
The UKBB has so far collected genotypes and deep clinical phenotypes for ~150,000 humans, with measures
including BMI, lipids, and other CVD risk factors. We will use BMI as an interaction term to test whether the
variants within lipid challenge-responsive regions in primary HWA are more likely to affect TGs in the ...

## Key facts

- **NIH application ID:** 9908159
- **Project number:** 5F31HL142180-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **Principal Investigator:** Kristina Marie Garske
- **Activity code:** F31 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $37,098
- **Award type:** 5
- **Project period:** 2018-04-12 → 2021-04-11

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9908159

## Citation

> US National Institutes of Health, RePORTER application 9908159, Integrating functional genomics in primary human adipocytes to investigate gene regulatory circuitry for obesogenic cardiovascular traits (5F31HL142180-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9908159. Licensed CC0.

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