# Transgenic and Geropathology Core

> **NIH NIH U19** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 2020 · $336,371

## Abstract

ABSTRACT – CORE B
The Transgenic and Geropathology Core, based at the University of Washington, will generate knockin or
knockout mice for Project 2 using Crisper/Cas technology, carrying the variants identified in Project 1. Dr.
Ladiges, in collaboration with Dr. Yousin Suh at Albert Einstein College of Medicine, has already generated a
mouse strain carrying a rare variant in IGF-R1 identified by Project 1. Also, the Core will assist Project 1 in
generating knockin or knockout cell lines for analysis of effects of the rare variants and miRNAs on growth,
signaling and responses to stress. In addition, the Core will provide histopathologic analysis of tissue aging in
the different mouse strains, for both Project 2 and Project 3, using an established Geropathology Grading
Platform (GGP). The GGP is based on a standard set of guidelines, which have been developed to 1) detect
the histological presence or absence of low impact lesions; and 2) determine the level of severity of high
impact lesions in organs from aged mice. The platform is designed to generate a numerical score for each
lesion in a specific organ, so that a total lesion score is obtained by adding each lesion score for that organ for
one mouse. Total lesion scores are averaged between all mice in a specific cohort to obtain a composite lesion
score (CLS) for that organ. The CLS will be used to investigate tissue effects of alterations in gene expression
(Project 2), and compare responses to drug treatment over time (Project 3). The core is vital to the success of
the projects and the overall success of the program.

## Key facts

- **NIH application ID:** 9916685
- **Project number:** 5U19AG056278-05
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Warren C LADIGES
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $336,371
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9916685

## Citation

> US National Institutes of Health, RePORTER application 9916685, Transgenic and Geropathology Core (5U19AG056278-05). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/9916685. Licensed CC0.

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