# Core C: Microarray Core Facility

> **NIH NIH P01** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 2020 · $306,465

## Abstract

SUMMARY
The Core C microarray core facility supports the proposed project aims to address the central hypothesis of the
program, as stated that Protein glycosylation and glycoprotein remodeling modulate the coagulopathy and
inflammation of sepsis. Core C provides unique technological capabilities and high throughput microarray
analyses of blood-derived experimental samples to most effectively achieve the research aims of all three
projects. Core C has developed a rapid and sensitive microarray-based analytical technology using
immobilized lectins to detect changes in glycan linkages in biological fluids. This lectin microarray has detected
the remodeling of glycoproteins in the blood during sepsis that includes the appearance of asialoglycoproteins
coincident with the expression of host and pathogen neuraminidases. The glycan linkages of circulating and
vascular glycoproteins including the proteoglycans have recently been found to play significant roles in the
pathogenesis of inflammation and coagulopathy during sepsis. Core C will undertake studies designed by the
projects to investigate these newly discovered changes to blood glycoproteins that are activated in the host
and which modulate the coagulopathy and inflammation of sepsis. In parallel, Core C will provide a custom
antibody-based microarray to detect changes in selected circulating proteins that are under study by the
projects as well as those known to participate in the onset of inflammation that can lead to extensive vascular
and organ damage during sepsis. Analyses provided by by Core C will compare and quantify these changes in
the blood during sepsis as well as in the non-microbial Systemic Inflammatory Response Syndrome. As
detailed in the Core C Research Strategy, Core C will accomplish lectin and antibody microarray analyses of
blood plasma samples from mice and humans, accomplish hematology data profiling of blood samples from
mice and humans, and determine pathogen colony forming unit measurements of blood samples when
requested. As with mouse plasma samples provided by the projects, Core C will analyze human plasma for
changes in glycan linkages among secreted proteins as well as compare the abundance of inflammatory
markers. Core C microarray assays include plasma measurements of heparan sulfate proteoglycans that have
recently been found to modulate the inflammation and coagulopathy in sepsis. Core C will enhance the rate of
progress of program research and facilitate research integration and synergies among the studies proposed.
Although analyses of blood and blood components remain the focus of Core C activity, the Core C core facility
can extend screening capabilities to other biological fluids if needed.

## Key facts

- **NIH application ID:** 9916810
- **Project number:** 5P01HL131474-05
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **Principal Investigator:** JAMEY MARTH
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $306,465
- **Award type:** 5
- **Project period:** — → 2021-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9916810

## Citation

> US National Institutes of Health, RePORTER application 9916810, Core C: Microarray Core Facility (5P01HL131474-05). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/9916810. Licensed CC0.

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