# Tau pathology in neurons, glia, and cell type-specific exosomes from cryopreserved AD cortex

> **NIH NIH R21** · UNIVERSITY OF CALIFORNIA LOS ANGELES · 2020 · $429,000

## Abstract

Project Summary
The present project has the goal of developing flow cytometry methods to analyze glia and neurons within
enzymatically dissociated cells from cryopreserved human cortex, and to simultaneously isolate cell-specific
extracelleular vesicles(EVs)/exosomes from the same tissue samples. As a proof of concept initial hypothesis,
we expect to observe stage-mediated tau increases in AD neurons and glia, and in EVs from the same
samples. An extensive literature documents the importance of synaptic tau propagation in Alzheimer's disease
and other tauopathies tau immunotherapy has shown efficacy in more than 13 studies. More recent studies
demonstrate a major role for microglia and possibly astrocytes in the spread of neuronal tau pathology, but to
our knowledge, no approach exists to analyze multiple brain cell types from the same human brain. Samples
will come from our bank, developed over decades, of more than 350 postmortem AD and aged control cortex
samples that are cryopreserved at the time of autopsy. A tauopathy mouse model that expresses human tau
will also be studied. Aim 1 will optimize flow cytometry methods to identify and isolate neurons, astrocytes and
microglia in dissociated cells from cryopreserved AD cortex. Aim 2 will isolate exosome/EVs from each AD
cortical sample at the time of dissociation, and isolate tau-bearing EVs of neuronal, astrocyte and microglial
origin. This proposal will directly address the contribution of the major brain cell types to tau progression in AD,
at the same time considering the role of exosomes from each cell type. The proposed protocols are highly
novel and will maximize the use of postmortem samples and our flow cytometry expertise.

## Key facts

- **NIH application ID:** 9917554
- **Project number:** 1R21AG063767-01A1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA LOS ANGELES
- **Principal Investigator:** Tina Bilousova
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $429,000
- **Award type:** 1
- **Project period:** 2020-01-15 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9917554

## Citation

> US National Institutes of Health, RePORTER application 9917554, Tau pathology in neurons, glia, and cell type-specific exosomes from cryopreserved AD cortex (1R21AG063767-01A1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9917554. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*