# Regulation of chromosome cohesion during cell cycle progression

> **NIH NIH R01** · OKLAHOMA MEDICAL RESEARCH FOUNDATION · 2020 · $121,184

## Abstract

Following DNA replication, the two identical copies of each chromosome are held together until
cell division by a process referred to as sister chromatid cohesion. Sister chromatid cohesion is
essential for accurate chromosome segregation, repair of DNA damage, the maintenance of
transcriptional programs, and normal development. Mutations in cohesion genes lead to birth defects
and are associated with genomic instability associated with certain cancers. Failures in cohesion are
thought to underlie the maternal age-related increase in aneuploid eggs, a major cause of birth
defects and spontaneous abortion.
 Sister chromatid cohesion is mediated by a large protein complex called cohesin. The activity of
this complex must be regulated to ensure that cohesin is laid down on chromatin and activated in a
way that is integrated with DNA replication and cell cycle progression. This regulation is
accomplished by the activities of several proteins that cooperate to regulate the stability of the
cohesin-chromatin interaction. Here we propose a series of experiments to define both how these
proteins, which we dub the “cohesin stability network”, are regulated during DNA replication and in
response to cell cycle progression. Using a combination of biochemical and cell biology approaches,
including assays in cultured cells and frog egg extracts, we will define the functional interactions
between the cohesin complex and this regulatory network. The results of these experiments will
provide critical insight into the mechanism by which cohesion is established, maintained, and
remodeled in vertebrate systems.

## Key facts

- **NIH application ID:** 9918789
- **Project number:** 3R01GM101250-07S1
- **Recipient organization:** OKLAHOMA MEDICAL RESEARCH FOUNDATION
- **Principal Investigator:** Susannah Rankin
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $121,184
- **Award type:** 3
- **Project period:** 2013-07-01 → 2022-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9918789

## Citation

> US National Institutes of Health, RePORTER application 9918789, Regulation of chromosome cohesion during cell cycle progression (3R01GM101250-07S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9918789. Licensed CC0.

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