# Protein/Structure Core

> **NIH NIH P01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2020 · $631,748

## Abstract

PROJECT SUMMARY: PROTEIN CORE
The Protein Core provides the technology, infrastructure and expertise required for the high-throughput
production of protein reagents to support the Ligand Discovery, Structure Determination and Functional
Annotation goals of the Program Project. The Protein Core will generate ~450 proteins per annum (native and
seleno-methionine substituted, as needed) for function and structure. Initial efforts will focus on the production
of Solute Binding Proteins (SBPs) and Ligand-responsive Transcriptional Regulators. Efforts will also include
“pathway-related” enzymes, as required for confirmation of metabolic pathway prediction by the Metabolism
and Modeling Projects. The Protein Core will exploit its great flexibility to respond to the changing needs of
the Program Project by providing access to the widest range of required proteins. This includes the generation
of oxygen sensitive proteins involved in amino acid and carbohydrate metabolism by exploiting the world's first
integrated system for high-throughput functional and structural biology of oxygen sensitive proteins, a
recapitulation of our entire high-throughput protein production and crystallization pipeline within an oxygen-free
environment.
In summary, we established a world class protein production resource which has supported the high-
throughput structural, DSF, activity-based screening and functional assay requirements for a number of large-
scale research programs and which will continue to do so for this Program Project.

## Key facts

- **NIH application ID:** 9918940
- **Project number:** 5P01GM118303-05
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** STEVEN C. ALMO
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $631,748
- **Award type:** 5
- **Project period:** — → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9918940

## Citation

> US National Institutes of Health, RePORTER application 9918940, Protein/Structure Core (5P01GM118303-05). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/9918940. Licensed CC0.

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