# Regulation of oxidative stress and vascular remodeling by EBP50

> **NIH NIH R01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2020 · $343,130

## Abstract

Chronic inflammation and oxidative stress are hallmarks of many age-related diseases, including
cardiovascular disease. There is a critical need to establish the molecular and cellular basis for the increased
and sustained inflammatory and oxidative status that leads to vascular disease. We have recently shown that
the scaffolding protein Ezrin Binding Protein 50 (EBP50, also known as NHERF1) is a key regulator of
systemic and vascular inflammation. Studies in mice and in cell systems show that inflammatory stimuli
promote EBP50 expression in macrophages and vascular smooth muscle cells (VSMC) and, conversely,
EBP50 increases inflammatory responses in these cells. Preliminary studies also indicate that EBP50
increases the generation of reactive oxygen species and potentiates oxidative stress in vessels. These findings
suggest a novel and pathologically relevant role for EBP50 in the control of both inflammation and oxidative
stress. We hypothesize that EBP50 promotes a chronic inflammatory and oxidative status that contributes to
atherosclerosis and vascular disease. Three specific aims are designed to test this hypothesis and elucidate
the molecular mechanisms underlying these novel actions of EBP50. In Aim 1 that molecular mechanisms by
which EBP50 regulates oxidative stress will be elucidated. Aim 2 will determine the actions of EBP50 that
regulate mitochondrial function and the responses to inflammatory and oxidative stimuli. In Aim 3, murine
models will be used to determine the effect of EBP50 on neointima formation. This research plan employs
complementary methods to define a novel mechanism by which EBP50 regulates inflammation and oxidative
stress. The long-term goal is to identify and exploit novel avenues for pharmacological intervention aimed at
limiting chronic inflammation and oxidative stress.

## Key facts

- **NIH application ID:** 9920603
- **Project number:** 5R01HL136382-04
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** ALESSANDRO BISELLO
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $343,130
- **Award type:** 5
- **Project period:** 2017-07-10 → 2022-03-10

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9920603

## Citation

> US National Institutes of Health, RePORTER application 9920603, Regulation of oxidative stress and vascular remodeling by EBP50 (5R01HL136382-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9920603. Licensed CC0.

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