# Core B - Metabolomics

> **NIH NIH P01** · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · 2020 · $222,200

## Abstract

SUMMARY – CORE B: METABOLOMICS 
The development of cancer is inherently linked to alterations in cellular metabolism. Exploration of these 
changes and the underlying regulatory mechanisms, with the goal of enabling new approaches for the 
diagnosis and treatment of melanoma, is a long-range objective of this P01 Program Project. The primary 
overall objective for Core B is to apply metabolic profiling methodology developed by core members for the 
comprehensive assessment of changes in metabolism upon altered ER homeostasis and mitochondrial 
function in melanoma, all processes that are tightly linked to central metabolism. Core B will fulfill this objective 
by a unique combination of methodology to comprehensively analyze metabolism, expertise in interpreting 
analytical results to explain the broader metabolic picture, and requisite experience in enzymology for detailed 
follow-up studies on metabolic enzymes. Central metabolism was already a focus for study in melanoma by the 
investigators of this grant in collaboration with the Core B leaders in the first phase of this P01 program. The 
Specific Aims of this core are as follows: (1) to provide comprehensive metabolic analytical support to all three 
collaborating research projects; (2) to characterize the central metabolism of the set of cell lines used in all 
research projects and Core C (melanoma screening and stratification core); and (3) to provide support to the 
research projects by assisting in the characterization of enzymes and enzymatic activities. The central method 
to be used is gas chromatography-mass spectrometry (GC-MS) analysis of polar and apolar metabolites in 
fractions of cells or medium derived from cultures labeled with stable isotopes (13C). This GC-MS technique 
yields data on metabolite quantities and also using isotope tracing, on metabolite origins and flux through 
metabolic pathways. Complemented with other methods to measure substrate uptake (YSI) and for oxygen 
consumption (Seahorse), it provides a comprehensive assessment of central metabolism. In general, further 
advances to this novel approach to metabolic analysis will also provide a model for future studies on cancer 
metabolism and cellular metabolism in general.

## Key facts

- **NIH application ID:** 9925086
- **Project number:** 5P01CA128814-10
- **Recipient organization:** SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
- **Principal Investigator:** ANDREI L OSTERMAN
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $222,200
- **Award type:** 5
- **Project period:** — → 2021-10-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9925086

## Citation

> US National Institutes of Health, RePORTER application 9925086, Core B - Metabolomics (5P01CA128814-10). Retrieved via AI Analytics 2026-06-14 from https://api.ai-analytics.org/grant/nih/9925086. Licensed CC0.

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