# Odor Coding in Piriform Cortex

> **NIH NIH R01** · DUKE UNIVERSITY · 2020 · $337,875

## Abstract

The ability to identify an odor is crucial for avoiding predators, obtaining food or finding a mate. The concerted
activity of distributed ensembles of neurons in piriform cortex (aPCx) is thought to lead directly to the formation
of an odor percept however the coding strategies used to represent different features of an odor stimulus, like
odor identity and odor intensity, remain poorly understood. aPCx consists of a heterogeneous population of
neurons that likely serve distinct roles in shaping odor representations and/or conveying this information to
downstream target areas. Little is known about how they actually do so as most investigations into cortical odor
coding were agnostic to cell type or target projection. Moreover, most previous studies were performed in
anesthetized animals, where both the cells' functions and consequent cortical representations may be dra-
matically altered. The objective here is to understand how different features of an odor stimulus are repre-
sented in diverse populations of aPCx neurons, and elucidate the underlying neural circuit mechanisms that
shape these representations. The approach used will be to record odor-evoked spiking in large ensembles of
functionally identified aPCx neurons in awake mice. The central hypothesis is that independent coding strate-
gies are used to represent odor identity and odor intensity, and that different types of aPCx cells play distinct
roles in shaping these representations. The rationale for these studies is that determining how odors are repre-
sented in aPCx of an awake, behaving animal is crucial for understanding olfactory system function. To this
end the following three aims are proposed: Aim 1: To determine how piriform cortex simultaneously rep-
resents odor identity and odor intensity. Preliminary studies suggest that odor identity is encoded in distrib-
uted ensembles of aPCx neurons, and these ensembles are largely concentration-invariant (i.e. a spatial iden-
tity code); and odor intensity is encoded by the synchrony of the ensembles activity (i.e. a temporal intensity
code). Aim 2: To determine odor responses in diverse subtypes of aPCx neurons, defined genetically,
by laminar organization or by distinct projection targets. In vivo optogenetic tagging will be used to identify
responses in defined subtypes of neurons. Odor responses in different subtypes of neurons are predicted to
reflect their specific roles in shaping the ensemble or the feature of the odor stimulus most relevant for different
target regions. Aim 3: To determine how recurrent circuitry shapes cortical odor representations. Output
from principal neurons will be unilaterally blocked, permitting simultaneous bilateral recordings of control and
“feedforward” circuits. Preliminary data suggest recurrent circuits dramatically shape the size, timecourse and
gain of odor responses in aPCx. This proposal is innovative because it deploys novel tools and methods to re-
cord odor-evoked activity in large...

## Key facts

- **NIH application ID:** 9928036
- **Project number:** 5R01DC015525-05
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Kevin Franks
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $337,875
- **Award type:** 5
- **Project period:** 2016-06-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9928036

## Citation

> US National Institutes of Health, RePORTER application 9928036, Odor Coding in Piriform Cortex (5R01DC015525-05). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9928036. Licensed CC0.

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