# Ribosomally Synthesized Proteins Incorporating Modified Dipeptides

> **NIH NIH R01** · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · 2020 · $293,550

## Abstract

Project Summary
The long term goals of this research involve modification of the peptidyltransferase (PTC) center in
bacterial ribosomes to enable the incorporation of very unusual amino acids not recognized by wild-
type ribosomes. In recent years, we have described the selection of modified ribosomes and their use
in incorporating D-amino acids, beta amino acids, dipeptides and dipeptidomimetric cassettes into
proteins. During the five years of proposed research, the focus of efforts will be on the introduction of
conformationally constrained cyclic dipeptides and nucleobase amino acids into specific proteins
(both as mono- and dipeptides), creating species that have novel properties enabling enhanced
analysis of protein function to be realized, as well as the analysis of specific conformational properties
essential for enzyme function and strategies for enhancing (and potentially modifying) protein-DNA
recognition.

## Key facts

- **NIH application ID:** 9928944
- **Project number:** 5R01GM121367-04
- **Recipient organization:** ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
- **Principal Investigator:** Sidney M. Hecht
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $293,550
- **Award type:** 5
- **Project period:** 2017-07-15 → 2021-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9928944

## Citation

> US National Institutes of Health, RePORTER application 9928944, Ribosomally Synthesized Proteins Incorporating Modified Dipeptides (5R01GM121367-04). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/9928944. Licensed CC0.

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