# Core B - Epigenetics and Bioinformatics Core

> **NIH NIH P01** · EMORY UNIVERSITY · 2020 · $388,394

## Abstract

Each of the projects within this PPG proposes aims that will determine the transcriptional and epigenetic
programs of plasma cell development and maintenance. To provide expertise, ensure standardized protocols,
integration of results and their subsequent analyses, and the sharing of data, the creation of an Epigenomics,
Sequencing and Bioinformatics Core (Core B) within this PPG is proposed. Core B will provide state-of-the-art
technologies, molecular biology expertise, and bioinformatic services that assess DNA methylation, chromatin
state and accessibility, and transcript expression through deep sequencing. Core B will be an independent
division within the Emory Integrated Genomics Core (EIGC), which is a full service genomics and
computational facility. This relationship will allow Core B to take advantage of the EIGC's computational
expertise, equipment, negotiated sequencing costs, and data storage. To serve the projects, four tasks are
proposed. Task 1: Provide uniform and quality sequence library sample preparation. Core B will create
high-quality sequencing libraries based on four technologies to derive an epigenetic program. RNA-seq will be
used to determine the transcriptome. Reduced Representation Bisulfite Sequencing (RRBS) will be used to
assess DNA methylation. The Assay for Transposase Accessible Chromatin (ATAC-seq) will determine
chromatin accessibility. Chromatin Immunoprecipitation-sequencing (ChIP-seq) will be used to determine
histone posttranslational modifications or transcription factor binding. Task 2: Determine library quality and
complexity and coordinate sequence runs. This task will identify library quality, determine the final
sequencing depth for each sample, and provide significant cost savings to the PPG by coordinating DNA
sequencing across projects. Task 3: Provide initial DNA sequence mapping, quality analysis, and data
storage and sharing. Core B will use an established pipeline for mapping of sequences to the respective
reference genome, provide long-term data storage, and facilitate sharing of processed datasets. Task 4:
Provide iterative bioinformatic computational analysis of datasets. A question driven, iterative
bioinformatics analysis will be used to derive epigenetic maps, features, metabolic processes, and
transcriptional circuitry associated with plasma differentiation and maintenance in each of the experimental
systems of the PPG. The iterative process will allow similar datasets (e.g., RRBS vs. RRBS) to be compared
and then integrated across technologies (e.g., RNA-seq and ATAC-seq). By combining all four technologies,
we will develop a multi-dimensional view of the epigenetic programming of plasma cells. Accordingly, Core B
will allow seamless integration of data across projects, allow systems comparisons between species, as well
as determine differences between healthy vs disease states to take place. Thus, Core B will provide a
common resource and analytical platform that will serve to facilita...

## Key facts

- **NIH application ID:** 9930036
- **Project number:** 5P01AI125180-05
- **Recipient organization:** EMORY UNIVERSITY
- **Principal Investigator:** JEREMY M. BOSS
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $388,394
- **Award type:** 5
- **Project period:** 2016-06-25 → 2022-05-11

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9930036

## Citation

> US National Institutes of Health, RePORTER application 9930036, Core B - Epigenetics and Bioinformatics Core (5P01AI125180-05). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/9930036. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*