# Transcriptomics and Repertoire Profiling

> **NIH NIH P01** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2020 · $481,750

## Abstract

CORE C: PROJECT SUMMARY
The main goal of this core is to establish and provide robust experimental procedures for profiling the
transcriptomes and immune repertoires of tissue-resident lymphocytes. We will employ innovative methods for
whole transcriptome and immune repertoire profiling in bulk populations, epigenomics, and single cell analysis.
For low-input, population-level RNA-Seq we will use our recently established protocol for high-sensitivity mRNA
capture and library construction. We will also provide bulk ATAC-Seq for measuring open chromatin. For single-
cell RNA-Seq, we will deploy our microfluidic system for large-scale single cell capture and cDNA barcoding as
well as a highly efficient plate-based method for whole transcriptome amplification. Finally, for dissecting the
clonal architecture of tissue-resident T and B cells, we will provide cutting-edge methods for T cell receptor and
antibody repertoire profiling.

## Key facts

- **NIH application ID:** 9931121
- **Project number:** 5P01AI106697-08
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Peter Alan Sims
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $481,750
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9931121

## Citation

> US National Institutes of Health, RePORTER application 9931121, Transcriptomics and Repertoire Profiling (5P01AI106697-08). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9931121. Licensed CC0.

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