# Scientific Core

> **NIH NIH P01** · STANFORD UNIVERSITY · 2020 · $248,163

## Abstract

Project Summary/Abstract
This Core will support the Projects with both standard MLR monitoring and flow cytometry, and
advanced technology including immune cell subset assays by CyTOF analysis, and T cell clonal
repertoire assays by high throughput sequencing of TCR genes from appropriate T cell
populations, and mRNA expression in DC populations by RNAseq. The Core will support both
preclinical models in Projects 2-3 by analyzing mouse cells, and the clinical study in Project 1 by
analyzing human cells. In the case of CyTOF, staining of cells for up to 40 targets
simultaneously using heavy metal conjugated mAbs and analysis by spectroscopy is expected
to lead to the identification of new subsets of adaptive and innate immune cells that will provide
additional insights into cells that contribute to tolerance in the preclinical and clinical studies. In
the case of high throughput sequencing, alloreactive T cell clones will be identified and purified
from pretransplant MLR cultures with recipient responder cells and donor stimulator cells. Clonal
frequencies will be determined in recipients before and after tolerance induction to search for
evidence of clonal deletion. In addition, the breadth of the T cell clonal repertoire will be
examined to study long term immune reconstitution.

## Key facts

- **NIH application ID:** 9931281
- **Project number:** 5P01HL075462-15
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** EDGAR G. ENGLEMAN
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $248,163
- **Award type:** 5
- **Project period:** — → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9931281

## Citation

> US National Institutes of Health, RePORTER application 9931281, Scientific Core (5P01HL075462-15). Retrieved via AI Analytics 2026-06-08 from https://api.ai-analytics.org/grant/nih/9931281. Licensed CC0.

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