# Spatiotemporal Epitranscriptome Editing Technology

> **NIH NIH R21** · CASE WESTERN RESERVE UNIVERSITY · 2020 · $602,140

## Abstract

Project Summary/ Abstract
 Dynamic chemical modifications on RNAs have been discovered to play critical regulatory roles in gene
regulation, cellular processes and human diseases including cancers. However, the definite roles of these
modifications and underlying mechanisms in their involvement in gene regulation and disease processes
remain elusive due to the complexity of epitranstriptomic regulation and the lack of proper enabling tools. To
address these limitations, we propose to develop new technologies that enable the editing of specific RNA
modifications, including N6-methyladenosine (m6A) and 5-methylcytosine (m5C), on specific endogenous
messenger RNA (mRNA) transcripts. We will combine the RNA-targeting CRISPR method and the chemically
induced proximity technology to achieve spatiotemporal specific epitranscriptome editing. We expect this new
technology will significantly facilitate the research of RNA epigenetics in cancer and may lead to the
development of new cancer therapies resulted from erroneous RNA modifications.

## Key facts

- **NIH application ID:** 9932038
- **Project number:** 1R21CA247638-01
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **Principal Investigator:** Fu-Sen Liang
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $602,140
- **Award type:** 1
- **Project period:** 2020-07-01 → 2023-12-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9932038

## Citation

> US National Institutes of Health, RePORTER application 9932038, Spatiotemporal Epitranscriptome Editing Technology (1R21CA247638-01). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/9932038. Licensed CC0.

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