# Kinetic Characterization of Hematopoietic Stem Cell Differentiation During Homeostasis And Pathological Conditions

> **NIH NIH F32** · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · 2020 · $65,310

## Abstract

Project Summary
 Hematopoiesis is the process in bone marrow by which all blood cells are generated throughout
organismal life. Normal hematopoiesis is impaired in multiple diseases including anemias, cytopenias, and
hematological malignancies such as leukemias and lymphomas. Little is known about the overall dynamics and
progenitor hierarchy of steady-state unperturbed hematopoiesis (as opposed to the transplant setting), and how
these are affected by oncogenic transformation or iatrogenic insults such as lineage ablation. Our overall goal is
to characterize in detail the in situ dynamics of physiological hematopoiesis and progenitor phenotypes at steady-
state and during adaptation to hematopoietic disruption. We propose to employ specific labeling and lineage
tracing of hematopoietic stem cells (HSC) with cellular indexing of transcriptomes and epitopes by single cell
sequencing (CITE-Seq) to accomplish three specific aims. The first is to characterize the intermediate progenitor
phenotypes and dynamics in an unbiased manner during steady-state hematopoiesis. In doing so, we will
construct a comprehensive and unbiased roadmap of hematopoietic differentiation. The second aim is to
investigate adaptation of hematopoiesis to leukemogenic perturbation. And lastly, the third aim is to elucidate
hematopoietic adaptation to the clinically relevant procedure of mature hematopoietic cell ablation. The proposed
studies will describe for the first time the kinetics of hematopoietic differentiation in a variety of physiological,
pathological and iatrogenic settings. To accomplish this goal, we will synergistically integrate cutting-edge
techniques in an innovative way. These studies would significantly advance the field of hematology, paving the
way for the development of novel therapies for maladies caused by disruption of normal hematopoietic
differentiation.

## Key facts

- **NIH application ID:** 9932122
- **Project number:** 5F32HL145997-02
- **Recipient organization:** NEW YORK UNIVERSITY SCHOOL OF MEDICINE
- **Principal Investigator:** Joseph Nicholas Pucella
- **Activity code:** F32 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $65,310
- **Award type:** 5
- **Project period:** 2019-04-01 → 2021-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9932122

## Citation

> US National Institutes of Health, RePORTER application 9932122, Kinetic Characterization of Hematopoietic Stem Cell Differentiation During Homeostasis And Pathological Conditions (5F32HL145997-02). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9932122. Licensed CC0.

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