# Transgenic Mouse/ES Shared Resource

> **NIH NIH P30** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2020 · $136,048

## Abstract

Transgenic Mouse/Embryonic Stem Cell Shared Resource: Project Summary/Abstract
For the past 22 years, the Transgenic Mouse/Embryonic Stem Cell Shared Resource (TMESCSR) has
provided a cornerstone for many scientific advances within the Vanderbilt Diabetes Research and Training
Center (VDRTC), and elsewhere. Not only have the TMESCSR-derived mice enabled hundreds of highly
impactful studies to be performed by VDRTC investigators, they have also been utilized by numerous other
non-VDRTC investigators who obtain these animals from public repositories. With the discovery of
CRISPR/Cas, which enables the derivation of mice with precisely targeted mutations in a rapid and efficient
manner, the TMESCSR made the strategic decision to discontinue its long-standing mouse ES cell (mESC)-
based gene targeting service. This action enabled the personnel supporting this resource to optimize and
deliver a new CRISPR/Cas-based service, while continuing to provide many other frequently utilized mouse-
based services. Together, the services provided by the TMESCSR enable VDRTC members to efficiently
derive mice with new genetic alterations, to maintain and cryopreserve existing mouse lines, and to derive new
lines from the vast number of mutant mESCs and frozen embryos or sperm that are now available from several
large-scale mutagenesis projects, including the Knock-out Mouse Project (KOMP). VDRTC support enables
the TMESCSR to import and optimize new technology, such as CRISPR/Cas, thereby assuring that VDRTC
investigators have continuing access to up-to-date strategies for deriving state-of-the-art mouse models. These
mouse models, in turn, enable VDRTC members to perform high quality studies to understand the
pathogenesis of diabetes, obesity and other endocrine diseases, and to perform preclinical testing of new
therapies for these diseases, utilizing the expertise in other VDRTC-supported cores (e.g. Cell Imaging Shared
Resource, Metabolic Physiology Shared Resource, Hormone Assay and Analytical Services Core).

## Key facts

- **NIH application ID:** 9933896
- **Project number:** 5P30DK020593-43
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** MARK A MAGNUSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $136,048
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9933896

## Citation

> US National Institutes of Health, RePORTER application 9933896, Transgenic Mouse/ES Shared Resource (5P30DK020593-43). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9933896. Licensed CC0.

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